Anti-Dishevelled 2抗体(ab22618)

概述

  • 产品名称
    Anti-Dishevelled 2抗体
    参阅全部 Dishevelled 2 一抗
  • 描述
    兔多克隆抗体to Dishevelled 2
  • 特异性
    ab22618 recognises dishevelled 2. It doesn't cross-react with dishevelled 1 or dishevelled 3.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    RGGEASGTSDGGP(C)

    conjugated to KLH by a MBS linker, corresponding to amino acids 633/645 of Human Dishevelled 2.

  • 阳性对照
    • Dishevelled 2 transfected HEK293 cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 10mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    ab22618 is a rabbit antiserum partially purified by caprylic acid and ammonium sulphate precipitation.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: 1/1000. Predicted molecular weight: 18 kDa.

    Note: dilute ab22618 to working strength using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
    • 序列相似性
      Belongs to the DSH family.
      Contains 1 DEP domain.
      Contains 1 DIX domain.
      Contains 1 PDZ (DHR) domain.
    • 结构域
      The DIX domain mediates homooligomerization.
    • 细胞定位
      Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Dishevelled 2 (homologous to Drosophila dsh) antibody
      • Dishevelled dsh homolog 2 antibody
      • dishevelled segment polarity protein 2 antibody
      • Dishevelled-2 antibody
      • Dishevelled2 antibody
      • DSH homolog 2 antibody
      • DVL 2 antibody
      • Dvl2 antibody
      • DVL2_HUMAN antibody
      • Segment polarity protein dishevelled homolog DVL 2 antibody
      • Segment polarity protein dishevelled homolog DVL-2 antibody
      • Segment polarity protein dishevelled homolog DVL2 antibody
      see all

    Anti-Dishevelled 2 antibody 图像

    • ab22618 staining Dishevelled 2 in murine rib cartilage by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 20% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa Fluor® conjugated donkey anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).

      See Abreview

    实验方案

    Anti-Dishevelled 2 antibody (ab22618)参考文献

    ab22618 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Rib cartilage)
    Specification
    Rib cartilage
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
    Username

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    提交于 Jan 25 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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