概述

  • 产品名称Anti-Dishevelled 2抗体
    参阅全部 Dishevelled 2 一抗
  • 描述
    兔多克隆抗体to Dishevelled 2
  • 特异性ab22616 recognises Dishevelled 2. It does not recognises Dishevelled 1 or Dishevelled 3.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    (C)GAGRTGRPEERAPES

    conjugated to KLH, corresponding to amino acids 600/614 of Human Dishevelled 2.

  • 阳性对照
    • Dishevelled 2 transfected HEK293 cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 10mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明ab22616 is a rabbit antiserum partially purified by caprylic acid and ammonium sulphate precipitation.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 79 kDa.
Note: dilute ab22616 to working concentration using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used).

靶标

  • 功能Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
  • 序列相似性Belongs to the DSH family.
    Contains 1 DEP domain.
    Contains 1 DIX domain.
    Contains 1 PDZ (DHR) domain.
  • 结构域The DIX domain mediates homooligomerization.
  • 细胞定位Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dishevelled 2 (homologous to Drosophila dsh) antibody
    • Dishevelled dsh homolog 2 antibody
    • dishevelled segment polarity protein 2 antibody
    • Dishevelled-2 antibody
    • Dishevelled2 antibody
    • DSH homolog 2 antibody
    • DVL 2 antibody
    • Dvl2 antibody
    • DVL2_HUMAN antibody
    • Segment polarity protein dishevelled homolog DVL 2 antibody
    • Segment polarity protein dishevelled homolog DVL-2 antibody
    • Segment polarity protein dishevelled homolog DVL2 antibody
    see all

Anti-Dishevelled 2 antibody 图像

  • All lanes : Anti-Dishevelled 2 antibody (ab22616) at 1/1000 dilution

    Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 79 kDa
    Observed band size : 102 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab22616 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22616, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Dishevelled 2 antibody (ab22616)参考文献

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Mir wurde vom Labor bestätigt, dass die Konzentration dieses Antikörpers leider nicht ermittelbar ist, da er nur teilweise aufgereinigt ist. Eine Proteinmessung würde nicht die Konzentration des Antikörpers messen, son...

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