概述

  • 产品名称
    Anti-Dishevelled 2抗体
    参阅全部 Dishevelled 2 一抗
  • 描述
    兔多克隆抗体to Dishevelled 2
  • 特异性
    ab22616 recognises Dishevelled 2. It does not recognises Dishevelled 1 or Dishevelled 3.
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    (C)GAGRTGRPEERAPES

    conjugated to KLH, corresponding to amino acids 600/614 of Human Dishevelled 2.

  • 阳性对照
    • Dishevelled 2 transfected HEK293 cell line.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 10mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    ab22616 is a rabbit antiserum partially purified by caprylic acid and ammonium sulphate precipitation.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 79 kDa.
Note: dilute ab22616 to working concentration using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used).

靶标

  • 功能
    Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
  • 序列相似性
    Belongs to the DSH family.
    Contains 1 DEP domain.
    Contains 1 DIX domain.
    Contains 1 PDZ (DHR) domain.
  • 结构域
    The DIX domain mediates homooligomerization.
  • 细胞定位
    Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dishevelled 2 (homologous to Drosophila dsh) antibody
    • Dishevelled dsh homolog 2 antibody
    • dishevelled segment polarity protein 2 antibody
    • Dishevelled-2 antibody
    • Dishevelled2 antibody
    • DSH homolog 2 antibody
    • DVL 2 antibody
    • Dvl2 antibody
    • DVL2_HUMAN antibody
    • Segment polarity protein dishevelled homolog DVL 2 antibody
    • Segment polarity protein dishevelled homolog DVL-2 antibody
    • Segment polarity protein dishevelled homolog DVL2 antibody
    see all

图片

  • All lanes : Anti-Dishevelled 2 antibody (ab22616) at 1/1000 dilution

    Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 79 kDa
    Observed band size : 102 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab22616 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22616, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Skalka N  et al. Carboxypeptidase E: a negative regulator of the canonical Wnt signaling pathway. Oncogene : (2012). Read more (PubMed: 22824791) »
  • Alten L  et al. Differential regulation of node formation, nodal ciliogenesis and cilia positioning by Noto and Foxj1. Development 139:1276-84 (2012). Read more (PubMed: 22357932) »

See all 2 Publications for this product

客户评价及客户问答

Vielen Dank für Ihren Anruf.
Mir wurde vom Labor bestätigt, dass die Konzentration dieses Antikörpers leider nicht ermittelbar ist, da er nur teilweise aufgereinigt ist. Eine Proteinmessung würde nicht die Konzentration des Antikörpers messen, son...

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