Anti-Dishevelled 2抗体(ab104438)

概述

  • 产品名称
    Anti-Dishevelled 2抗体
    参阅全部 Dishevelled 2 一抗
  • 描述
    兔多克隆抗体to Dishevelled 2
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 650-700 of Human Dishevelled 2 (NP_004413.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab104438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 79 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
  • 序列相似性
    Belongs to the DSH family.
    Contains 1 DEP domain.
    Contains 1 DIX domain.
    Contains 1 PDZ (DHR) domain.
  • 结构域
    The DIX domain mediates homooligomerization.
  • 细胞定位
    Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dishevelled 2 (homologous to Drosophila dsh) antibody
    • Dishevelled dsh homolog 2 antibody
    • dishevelled segment polarity protein 2 antibody
    • Dishevelled-2 antibody
    • Dishevelled2 antibody
    • DSH homolog 2 antibody
    • DVL 2 antibody
    • Dvl2 antibody
    • DVL2_HUMAN antibody
    • Segment polarity protein dishevelled homolog DVL 2 antibody
    • Segment polarity protein dishevelled homolog DVL-2 antibody
    • Segment polarity protein dishevelled homolog DVL2 antibody
    see all

图片

  • All lanes : Anti-Dishevelled 2 antibody (ab104438) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 79 kDa


    Exposure time : 3 minutes
  • Detection of Dishevelled 2 in immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded on gel) using ab104438 at 3 µg/mg lysate for IP, and at 1 µg/ml for the subsequent western blot.

    Detection: chemiluminescence with exposure time of 30 seconds.

文献

ab104438 has not yet been referenced specifically in any publications.

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