概述

  • 产品名称Anti-Dis3抗体
  • 描述
    兔多克隆抗体to Dis3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Schizosaccharomyces pombe
  • 免疫原

    Recombinant fragment corresponding to Schizosaccharomyces pombe Dis3.
    Database link: P37202

  • 阳性对照
    • S. pombe cell lysates transformed with vector or plasmids carrying truncated genes; S.pombe cells.

性能

应用

Our Abpromise guarantee covers the use of ab64289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/300. Predicted molecular weight: 110 kDa.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性Dis 3 is a catalytic component of the exosome 3'->5' exoribonuclease complex required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA and for mRNA decay. Has a 3'-5' exonuclease activity. Implicated in mitotic control. Essential for cell division and spore germination. May be involved in regulating protein dephosphorylation during mitosis.
  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
    • 别名
      • 3'-5' exoribonuclease subunit Dis3 antibody
      • Chromosome disjunction protein 3 antibody
      • Exosome complex exonuclease dis3 antibody
      • Mitotic control protein dis3 antibody
      • Ribosomal RNA processing protein 44 antibody
      • rrp44 antibody
      see all

    Anti-Dis3 antibody 图像

    • Immunfluorescent analysis of S. pombe cells labeling Dis3 with ab64289 (right panel). DAPI stain for chromosomal DNA (left panel).

    • All lanes : Anti-Dis3 antibody (ab64289) at 1/100 dilution

      Lane 1 : S. pombe cell lysate transformed with the vector
      Lane 2 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (172)
      Lane 3 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (A)
      Lane 4 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (B)
      Lane 5 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (C)
      Lane 6 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (E)


      Predicted band size : 110 kDa

      Polypeptides of expected molecular masses were detected.

    Anti-Dis3 antibody (ab64289)参考文献

    This product has been referenced in:
    • Kinoshita N  et al. The fission yeast dis3+ gene encodes a 110-kDa essential protein implicated in mitotic control. Mol Cell Biol 11:5839-47 (1991). Read more (PubMed: 1944266) »

    See 1 Publication for this product

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