概述

  • 产品名称
  • 描述
    兔多克隆抗体to DIS
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 100 and 150 of human DIS. (Q8IX12).

  • 阳性对照
    • Nuclear extract from HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab70244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    IP: Use at 1-10µg/mg of lysate.
    WB: 1/1000 - 1/5000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation.
    • 组织特异性
      Expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors.
    • 序列相似性
      Contains 1 SAP domain.
    • 翻译后修饰
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位
      Cytoplasm > perinuclear region.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CARP-1 antibody
      • Carp1 antibody
      • Ccar1 antibody
      • CCAR1_HUMAN antibody
      • Cell cycle and apoptosis regulatory protein 1 antibody
      • cell division cycle and apoptosis regulator 1 antibody
      • Cell division cycle and apoptosis regulator protein 1 antibody
      • Death inducer with SAP domain antibody
      • FLJ10590 antibody
      • FLJ10839 antibody
      • FLJ13376 antibody
      • FLJ20734 antibody
      • MGC7730 antibody
      • RP11-437A18.1 antibody
      see all

    图片

    • All lanes : Anti-DIS antibody (ab70244) at 1 µg/ml

      Lane 1 : Nuclear extract from HeLa cells at 15 µg
      Lane 2 : Nuclear extract from HeLa cells at 45 µg


      Predicted band size : 132 kDa
      Observed band size : 132 kDa


      Exposure time : 1 minute

    文献

    This product has been referenced in:
    • Mizuta S  et al. CCAR1/CoCoA pair-mediated recruitment of the Mediator defines a novel pathway for GATA1 function. Genes Cells 19:28-51 (2014). Read more (PubMed: 24245781) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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