概述

  • 产品名称Anti-DIS抗体
    参阅全部 DIS 一抗
  • 描述
    兔多克隆抗体to DIS
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between N terminal residues 1 and 50 of human DIS. (SwissProt Q8IX12)

  • 阳性对照
    • Nuclear extract from HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab70243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/15000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
IP Use at 1-10 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation.
  • 组织特异性Expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors.
  • 序列相似性Contains 1 SAP domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CARP-1 antibody
    • Carp1 antibody
    • Ccar1 antibody
    • CCAR1_HUMAN antibody
    • Cell cycle and apoptosis regulatory protein 1 antibody
    • cell division cycle and apoptosis regulator 1 antibody
    • Cell division cycle and apoptosis regulator protein 1 antibody
    • Death inducer with SAP domain antibody
    • FLJ10590 antibody
    • FLJ10839 antibody
    • FLJ13376 antibody
    • FLJ20734 antibody
    • MGC7730 antibody
    • RP11-437A18.1 antibody
    see all

Anti-DIS antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling DIS with ab70243 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DIS antibody (ab70243) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 132 kDa


    Exposure time : 10 seconds
  • Immunoprecipitation analysis of whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells (lanes 1 and 2). ab70243 was used for IP at 6 µg/mg lysate. For western blot ab70243 was used at 1 µg/ml. Lane 3 - control IgG.

Anti-DIS antibody (ab70243)参考文献

ab70243 has not yet been referenced specifically in any publications.

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