Anti-DIS抗体(ab70243)
Key features and details
- Rabbit polyclonal to DIS
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-DIS抗体
参阅全部 DIS 一抗 -
描述
兔多克隆抗体to DIS -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan -
免疫原
Synthetic peptide corresponding to a region between N terminal residues 1 and 50 of human DIS. (SwissProt Q8IX12)
-
阳性对照
- Nuclear extract from HeLa cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Tris-citrate/phosphate buffer, pH 7 to 8 -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70243于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/5000 - 1/15000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
|
|
IP |
Use at 1-10 µg/mg of lysate.
|
|
IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/5000 - 1/15000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa). |
IP
Use at 1-10 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation. -
组织特异性
Expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors. -
序列相似性
Contains 1 SAP domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm > perinuclear region. - Information by UniProt
-
数据库链接
- Entrez Gene: 423692 Chicken
- Entrez Gene: 450498 Chimpanzee
- Entrez Gene: 767587 Cow
- Entrez Gene: 479230 Dog
- Entrez Gene: 55749 Human
- Entrez Gene: 67500 Mouse
- Entrez Gene: 361849 Rat
- Omim: 612569 Human
see all -
别名
- CARP-1 antibody
- Carp1 antibody
- Ccar1 antibody
see all
图片
-
All lanes : Anti-DIS antibody (ab70243) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 132 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling DIS with ab70243 at 1/1000 (1µg/ml). Detection: DAB.
-
Immunoprecipitation analysis of whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells (lanes 1 and 2). ab70243 was used for IP at 6 µg/mg lysate. For western blot ab70243 was used at 1 µg/ml. Lane 3 - control IgG.
实验方案
数据表及文件
-
Datasheet download
文献 (2)
ab70243 被引用在 2 文献中.
- Fei X et al. TRIM22 orchestrates the proliferation of GBMs and the benefits of TMZ by coordinating the modification and degradation of RIG-I. Mol Ther Oncolytics 26:413-428 (2022). PubMed: 36159777
- Li D et al. RP11-284F21.9 promotes lung carcinoma proliferation and invasion via the regulation of miR-627-3p/CCAR1. Oncol Rep 44:1638-1648 (2020). PubMed: 32945522