Anti-DIRAS2抗体(ab67430)

概述

  • 产品名称
    Anti-DIRAS2抗体
  • 描述
    小鼠多克隆抗体to DIRAS2
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Full length DIRAS2 protein (Human)

  • 阳性对照
    • DIRAS2 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab67430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
WB
  • 应用说明
    WB: 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      DIRAS2 displays low GTPase activity and exist predominantly in the GTP-bound form. It is highly expressed in the brain.
    • 细胞定位
      Cell membrane; Lipid-anchor; Cytoplasmic side
    • 数据库链接
    • 别名
      • Di Ras2 antibody
      • DIRAS family, GTP binding RAS like 2 antibody
      • DIRAS2 antibody
      • Distinct subgroup of the Ras family member 2 antibody
      • GTP-binding protein Di-Ras2 antibody
      see all

    图片

    • All lanes : Anti-DIRAS2 antibody (ab67430) at 1/500 dilution

      Lane 1 : DIRAS2 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 22 kDa
      Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    • ab67430 staining DIRAS2 in rat brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
      Tissue was fixed in paraformaldehyde, a heat mediated antigen retrieval step was performed using Tris-EDTA pH9, samples were then blocked using 5% serum and then incubated with ab67430 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted biotin conjugated goat polyclonal.

      See Abreview

    文献

    ab67430 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA, PH 9
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    提交于 Oct 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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