Anti-DIRAS2抗体(ab67430)

概述

  • 产品名称Anti-DIRAS2抗体
  • 描述
    小鼠多克隆抗体to DIRAS2
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Full length DIRAS2 protein (Human)

  • 阳性对照
    • DIRAS2 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab67430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
WB
  • 应用说明WB: 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性DIRAS2 displays low GTPase activity and exist predominantly in the GTP-bound form. It is highly expressed in the brain.
    • 细胞定位Cell membrane; Lipid-anchor; Cytoplasmic side
    • 数据库链接
    • 别名
      • Di Ras2 antibody
      • DIRAS family, GTP binding RAS like 2 antibody
      • DIRAS2 antibody
      • Distinct subgroup of the Ras family member 2 antibody
      • GTP-binding protein Di-Ras2 antibody
      see all

    Anti-DIRAS2 antibody 图像

    • All lanes : Anti-DIRAS2 antibody (ab67430) at 1/500 dilution

      Lane 1 : DIRAS2 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 22 kDa
      Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    • ab67430 staining DIRAS2 in rat brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
      Tissue was fixed in paraformaldehyde, a heat mediated antigen retrieval step was performed using Tris-EDTA pH9, samples were then blocked using 5% serum and then incubated with ab67430 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted biotin conjugated goat polyclonal.

      See Abreview

    Anti-DIRAS2 antibody (ab67430)参考文献

    ab67430 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (brain)
    Specification brain
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA, PH 9
    Permeabilization No
    Blocking step Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 Oct 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"