Anti-Diphtheria Toxin A subunit抗体[7F2] (ab8310)

概述

  • 产品名称
    Anti-Diphtheria Toxin A subunit抗体[7F2]
    参阅全部 Diphtheria Toxin A subunit 一抗
  • 描述
    小鼠单克隆抗体[7F2] to Diphtheria Toxin A subunit
  • 特异性
    This antibody does not react with the free B subunit of Diphtheria toxin.
  • 经测试应用
    适用于: ELISAmore details
  • 免疫原

    Full length protein.

  • 表位
    This antibody reacts with the epitope exposed on the free A subunit and on the whole Diphtheria toxin molecule.
  • 常规说明

    Detection of Diphtheria toxin A subunit

    For a recombinant antibody to Diphtheria Toxin, please see - Ab209329

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

应用

Our Abpromise guarantee covers the use of ab8310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [8A4] to Diphtheria Toxin A subunit (ab8308). Use as detection antibody.

靶标

  • 相关性
    Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B.
  • 别名
    • Diphtheria Toxin antibody

文献

This product has been referenced in:
  • Buch T  et al. A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat Methods 2:419-26 (2005). Read more (PubMed: 15908920) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Sample
Escherichia coli Recombinant protein (supernatant)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
supernatant
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 30 2016

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in Western blotting. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get...

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Dear Masanori ab8310 - This antibody reacts with the epitope exposed on the free A subunit and on the whole Diphtheria toxin molecule. ab8308 - this has not been fully characterised. This is currently the only information that we have.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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