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Our Abpromise guarantee covers the use of ab77481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.05 µg/ml. Predicted molecular weight: 31 kDa.Can be blocked with DIO2 peptide (ab131396). Preliminary experiments gave an approx 65-70 kDa band in human heart and thyroid lysates after 0.05 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.5 kDa according to NP_000784.2 and of 34.6 kDa according to NP_001007024.1. The 65-70 kDa band was successfully blocked by incubation with the immunizing peptide.|
|IHC-Fr||Use a concentration of 2 - 3 µg/ml. Tissue sections were PFA fixed.|
ab77481 staining DIO2 in mouse Brain (Hippocampus) tissue section by Immunohistochemistry (Frozen sections). Tissue underwent antigen retrieval in microwave with citrate buffer (pH 4.5). The HRP-staining procedure was used for detection.
ab77481 staining DIO2 in DIO2 Brain (Hypothalamus) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/1000) for 48 hours at 4°C. A Biotinylated Horse Anti-Goat IgG polyclonal antibody (1/400) was used as the secondary antibody.
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