概述

  • 产品名称
  • 描述
    山羊多克隆抗体to DIO2
  • 经测试应用
    适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Goat, Chicken, Cow, Human, Pig
  • 免疫原

    Synthetic peptide:

    EVKKHQNQEDRC

    (Human) from the internal region of the protein sequence according to NP_000784.2; NP_054644.1; NP_001007024.1.

  • 阳性对照
    • Human heart and thyroid lysates

性能

应用

Our Abpromise guarantee covers the use of ab77481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.05 µg/ml. Predicted molecular weight: 31 kDa.Can be blocked with DIO2 peptide (ab131396). Preliminary experiments gave an approx 65-70 kDa band in human heart and thyroid lysates after 0.05 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.5 kDa according to NP_000784.2 and of 34.6 kDa according to NP_001007024.1. The 65-70 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC-Fr Use a concentration of 2 - 3 µg/ml. Tissue sections were PFA fixed.

靶标

图片

  • ab77481 staining DIO2 in mouse Brain (Hippocampus) tissue section by Immunohistochemistry (Frozen sections). Tissue underwent antigen retrieval in microwave with citrate buffer (pH 4.5). The HRP-staining procedure was used for detection.

  • ab77481 staining DIO2 in DIO2 Brain (Hypothalamus) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/1000) for 48 hours at 4°C. A Biotinylated Horse Anti-Goat IgG polyclonal antibody (1/400) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Shu L  et al. A-FABP mediates adaptive thermogenesis by promoting intracellular activation of thyroid hormones in brown adipocytes. Nat Commun 8:14147 (2017). WB . Read more (PubMed: 28128199) »
  • Wang YY  et al. Up-regulation of type 2 iodothyronine deiodinase in dilated cardiomyopathy. Cardiovasc Res : (2010). WB ; Mouse . Read more (PubMed: 20453157) »

See all 2 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Sample
Red sided garter snake Tissue sections (Brain (Hypothalamus))
Permeabilization
No
Specification
Brain (Hypothalamus)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 06 2017

Thank you for your patience. My colleague has been able to add the following peptides to our catalog:

Ab77481 corresponding peptide Ab131396

Ab102926 corresponding peptide Ab131397

Ab22835 corresponding peptide AB131398 ...

Read More

Thank you for your reply.

I will be working on adding these peptides to our catalog, and it may take a couple of days.

Regardingthe TSH beta antibody ab80784, the immunogen was purified human TSH beta. We don't have the beta subuni...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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