概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to DIMT1L
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Full length human DIMT1L (NP_055288).

  • 阳性对照
    • Rat brain tissue lysate and DIMT1L transfected 293T cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

靶标

  • 功能
    Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.
  • 序列相似性
    Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.
  • 细胞定位
    Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase antibody
    • 18S rRNA dimethylase antibody
    • DIM1 antibody
    • DIM1 dimethyladenosine transferase 1 homolog (S. cerevisiae) antibody
    • DIM1 dimethyladenosine transferase 1 homolog antibody
    • DIM1 dimethyladenosine transferase 1 like antibody
    • DIM1 dimethyladenosine transferase 1-like antibody
    • Dimethyladenosine transferase antibody
    • DIMT1 antibody
    • DIMT1_HUMAN antibody
    • DIMT1L antibody
    • HSA9761 antibody
    • HUSSY5 antibody
    • N''-adenosyl(rRNA) dimethyltransferase antibody
    • Probable 18S rRNA (adenine(1779) N(6)/adenine(1780) N(6)) dimethyltransferase antibody
    • Probable 18S rRNA dimethylase antibody
    • Probable dimethyladenosine transferase antibody
    • Probable S adenosylmethionine 6 N',N' adenosyl(rRNA) dimethyltransferase antibody
    • S adenosylmethionine 6 N',N' adenosyl(rRNA) dimethyltransferase antibody
    • S-adenosylmethionine-6-N'' antibody
    see all

Anti-DIMT1L antibody 图像

  • Anti-DIMT1L antibody (ab69434) at 1/500 dilution + rat brain tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
  • All lanes : Anti-DIMT1L antibody (ab69434) at 1/500 dilution

    Lane 1 : DIMT1L transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 36 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-DIMT1L antibody (ab69434)参考文献

This product has been referenced in:
  • Zorbas C  et al. The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis. Mol Biol Cell 26:2080-95 (2015). ICC/IF . Read more (PubMed: 25851604) »

See 1 Publication for this product

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