Anti-Dihydropyrimidinase抗体(ab121844)

概述

  • 产品名称Anti-Dihydropyrimidinase抗体
    参阅全部 Dihydropyrimidinase 一抗
  • 描述
    兔多克隆抗体to Dihydropyrimidinase
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: TISRGKVVYE AGVFSVTAGD GKFIPRKPFA EYIYKRIKQR DRTCTPTPVE RAPYKGEVAT LKSRVTKEDA TAG, corresponding to amino acids 440-512 of Human Dihydropyrimidinase. (Q14117)

  • 阳性对照
    • Human kidney tissue; Dihydropyrimidinase over-expression HEK293T lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab121844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

  • 功能Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyzes the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate.
  • 组织特异性Liver and kidney.
  • 疾病相关Defects in DPYS are the cause of dihydropyrimidinase deficiency (DHPD) [MIM:222748]. DHPD is an autosomal recessive disorder characterized by dihydropyrimidinuria and associated with a variable clinical phenotype: epileptic or convulsive attacks, dysmorphic features and severe developmental delay, and congenital microvillous atrophy.
  • 序列相似性Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • 翻译后修饰Carbamylation allows a single lysine to coordinate two zinc ions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DHP antibody
    • DHPase antibody
    • Dihydropyrimidinase antibody
    • Dihydropyrimidine amidohydrolase antibody
    • Dpys antibody
    • DPYS_HUMAN antibody
    • Hydantoinase antibody
    see all

Anti-Dihydropyrimidinase antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in vesicles and centrosome. Recommended concentration of ab121844 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121844 at 1/500 dilution staining Dihydropyrimidinase in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • All lanes : Anti-Dihydropyrimidinase antibody (ab121844) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

    Developed using the ECL technique

Anti-Dihydropyrimidinase antibody (ab121844)参考文献

ab121844 has not yet been referenced specifically in any publications.

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