Anti-Dihydrofolate reductase (DHFR)抗体(ab49881)

概述

  • 产品名称Anti-Dihydrofolate reductase (DHFR)抗体
    参阅全部 Dihydrofolate reductase (DHFR) 一抗
  • 描述
    兔多克隆抗体to Dihydrofolate reductase (DHFR)
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Hamster, Human
    预测可用于: Mouse, Rat, Cow, Pig
  • 免疫原

    Synthetic peptide:

    PLRNEFRYFQRMTT

    , corresponding to N terminal amino acids 27-40 of Human Dihydrofolate reductase (DHFR) conjugated to KLH by a Cysteine residue linker.

性能

应用

Our Abpromise guarantee covers the use of ab49881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 21 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

Anti-Dihydrofolate reductase (DHFR) antibody 图像

  • All lanes : Anti-Dihydrofolate reductase (DHFR) antibody (ab49881) at 0.5 µg/ml

    Lane 1 : recombinant DHFR at 0.2 µg
    Lane 2 : recombinant DHFR at 0.1 µg
    Lane 3 : recombinant DHFR at 0.05 µg
    Lane 4 : recombinant DHFR at 0.025 µg
    Lane 5 : recombinant DHFR at 0.0125 µg

    Developed using the ECL technique

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
  • ab49881 at a 1/30 dilution staining Dihydrofolate reductase (DHFR) in human squamous cell carcinoma tissue by Immunohistochemistry/ Formalin fixed, paraffin embedded, incubated for 12 hours at 4°C. Heat mediated antigen retrieval step performed using EDTA pH 9.0. Blocked with 1% milk for 30 minutes at 25°C. Secondary used undiluted polyclonal Goat anti-rabbit IgG conjugated to biotin.

    See Abreview

Anti-Dihydrofolate reductase (DHFR) antibody (ab49881)参考文献

This product has been referenced in:
  • Anderson DD  et al. Serine hydroxymethyltransferase anchors de novo thymidylate synthesis pathway to nuclear lamina for DNA synthesis. J Biol Chem 287:7051-62 (2012). IP ; Human . Read more (PubMed: 22235121) »
  • Martin SA  et al. Methotrexate induces oxidative DNA damage and is selectively lethal to tumour cells with defects in the DNA mismatch repair gene MSH2. EMBO Mol Med 1:323-37 (2009). WB . Read more (PubMed: 20049736) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (squamous cell carcinoma)
Specification squamous cell carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Nov 30 2009

Application Western blot
Sample Human Cell lysate - whole cell (human fibroblast)
Loading amount 10000 cells
Specification human fibroblast
Gel Running Conditions Reduced Denaturing (4-20% acrylamide SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 08 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells)
Loading amount 20 µg
Specification Chinese Hamster Ovary cells
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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提交于 Nov 07 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (head/neck tumor)
Specification head/neck tumor
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5%
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提交于 Jan 03 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"