Anti-Digoxigenin抗体[BT.21H8] (Biotin) (ab419)

概述

  • 产品名称
    Anti-Digoxigenin抗体[BT.21H8] (Biotin)
    参阅全部 Digoxigenin 一抗
  • 描述
    小鼠单克隆抗体[BT.21H8] to Digoxigenin (Biotin)
  • 偶联物
    Biotin
  • 经测试应用
    适用于: WB, ELISA, IHC-P, IHC-Fr, IHC-FoFrmore details
  • 免疫原

    Synthetic peptide corresponding to Digoxigenin.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Thimerosal
    Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯化说明
    Purified biotin conjugate.
  • 克隆
    单克隆
  • 克隆编号
    BT.21H8
  • 骨髓瘤
    unknown
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/5000.
ELISA 1/1000 - 1/10000.
IHC-P 1/200 - 1/2000.
IHC-Fr 1/200 - 1/2000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21813679

靶标

  • 相关性
    Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
  • 别名
    • 1672-46-4 antibody
    • BRN 0096479 antibody
    • DIG antibody
    • EINECS 216-806-2 antibody
    • HSDB 7108 antibody
    • Lanadigenin antibody
    • Lanadigigenin antibody
    • ST056392 antibody
    see all

文献

This product has been referenced in:
  • Heine S  et al. CNGA3: A Target of Spinal Nitric Oxide/cGMP Signaling and Modulator of Inflammatory Pain Hypersensitivity. J Neurosci 31:11184-92 (2011). IHC-FoFr . Read more (PubMed: 21813679) »
  • Silva VA  et al. Expression and cellular localization of microRNA-29b and RAX, an activator of the RNA-dependent protein kinase (PKR), in the retina of streptozotocin-induced diabetic rats. Mol Vis 17:2228-40 (2011). In situ hybridization . Read more (PubMed: 21897745) »

See all 3 Publications for this product

客户评价及客户问答

Vielen Dank für Ihre Email. Es tut mir leid zu hören, dass Ihr versuch nicht optimal läuft.

Wenn ich Sie richtig verstehen haben sie den sekundären Antikörper (ab99900) mit einem anderen primären Antikörper ...

Read More

Vielen Dank für Ihre Antwort.

Es freut mich zu hören, dass das Experiment mit einem unkonjugierten Antikörperfunktioniert hat.

Leider ist es nicht möglich die Konzentration von ab420 zu bestimmen. Bei ab420 hande...

Read More

Vielen Dank für Ihre Anfrage.

Wenn Antikörper mit Biotin gekoppelt werden, wird das meist über freie Anime getan. Das bedeutet das Biotin über den ganzen Antikörper verteilt ist. Die Antikörper werden dann darauf...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (thymus, eye)
Specification
thymus, eye
Fixative
Paraformaldehyde
Permeabilization
Yes - tritonX-100 10min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Lampros Panagis

Verified customer

提交于 Feb 10 2011

Thank you for your enquiry. We do not provide MS data sheets for our products. All necessary information is located on the product datasheet (which can be viewed online or was shipped with the product).

We are sorry to inform you that this data is not available.

My apologies ab419 quite obivously is biotinylated. My only excuse is that for some reason our internal system isn't showing the conjugate when I search. The antibody is guaranteed for what it says on the datasheet i.e tested applications, species etc ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册