概述

  • 产品名称Anti-Dicer抗体
    参阅全部 Dicer 一抗
  • 描述
    小鼠多克隆抗体to Dicer
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
    预测可用于: a wide range of other species
  • 免疫原

    Vector coding for a partial recombinant fusion protein (His tagged), corresponding to internal sequence amino acids 2044-2063 of Fruit fly (Drosophila melanogaster) Dicer (Dicer 1) Target sequence used to make the antibody:

    TRHLYEDPRQHSPGALTDLR

    .

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 255 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
  • 疾病相关Pleuropulmonary blastoma
    Goiter multinodular 1, with or without Sertoli-Leydig cell tumors
    Rhabdomyosarcoma, embryonal, 2
    DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).
  • 序列相似性Belongs to the helicase family. Dicer subfamily.
    Contains 1 Dicer dsRNA-binding fold domain.
    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PAZ domain.
    Contains 2 RNase III domains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
    • 别名
      • DCR antibody
      • DCR1 antibody
      • Dicer 1 ribonuclease type III antibody
      • Dicer antibody
      • DICER_HUMAN antibody
      • DICER1 antibody
      • Double-strand-specific ribonuclease antibody
      • Endoribonuclease Dicer antibody
      • Helicase MOI antibody
      • Helicase with RNase motif antibody
      • HERNA antibody
      • KIAA0928 antibody
      • MNG1 antibody
      • RMSE2 antibody
      see all

    Anti-Dicer antibody 图像

    • All lanes : Anti-Dicer antibody (ab52680) at 1/1000 dilution

      Lane 1 : Total protein extract from E coli with 50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen.
      Lane 2 : Total protein extract from E coli with 50ng to 500ng of the antigen (recombinant fusion protein containing a His tag).

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM, HRP conjugated, at 1/5000 dilution

      Predicted band size : 255 kDa

    Anti-Dicer antibody (ab52680)参考文献

    This product has been referenced in:
    • Mukherjee S & Hanley KA RNA interference modulates replication of dengue virus in Drosophila melanogaster cells. BMC Microbiol 10:127 (2010). WB ; Drosophila melanogaster . Read more (PubMed: 20420715) »

    See 1 Publication for this product

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