概述

  • 产品名称
  • 描述
    兔多克隆抗体to Dicer
  • 特异性
    There are multiple bands in the blot shown, though there is a band at the appropriate predicted size (255 kDa).
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Synthetic peptide derived from residues 900 - 1000 Dicer (Dcr1).

    .

  • 阳性对照
    • This antibody gave a positive signal in drosophila embryo lysate.

性能

应用

Our Abpromise guarantee covers the use of ab4735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 255 kDa (predicted molecular weight: 255 kDa).

靶标

  • 功能
    Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
  • 疾病相关
    Pleuropulmonary blastoma
    Goiter multinodular 1, with or without Sertoli-Leydig cell tumors
    Rhabdomyosarcoma, embryonal, 2
    DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).
  • 序列相似性
    Belongs to the helicase family. Dicer subfamily.
    Contains 1 Dicer dsRNA-binding fold domain.
    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PAZ domain.
    Contains 2 RNase III domains.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
    • 别名
      • DCR antibody
      • DCR1 antibody
      • Dicer 1 ribonuclease III antibody
      • Dicer 1 ribonuclease type III antibody
      • Dicer antibody
      • DICER_HUMAN antibody
      • DICER1 antibody
      • Double-strand-specific ribonuclease antibody
      • Endoribonuclease Dicer antibody
      • Helicase MOI antibody
      • Helicase with RNase motif antibody
      • HERNA antibody
      • KIAA0928 antibody
      • MNG1 antibody
      • RMSE2 antibody
      see all

    图片

    • Lanes 1 - 2 : Anti-Dicer antibody (ab4735) at 1/500 dilution
      Lanes 3 - 4 : Anti-Dicer antibody (ab4735) at 1/1000 dilution

      Lane 1 : 5 ug of drosophila embryo lysate.
      Lane 2 : 5 ug of drosophila embryo lysate.
      Lane 3 : 5 ug of drosophila embryo lysate.
      Lane 4 : 5 ug of drosophila embryo lysate.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 255 kDa
      Observed band size : 255 kDa
      Additional bands at : 170 kDa (possible cleavage fragment,cross reactivity).

      Exposure time : 30 seconds

      Rabbit polyclonal to Dicer 1 (ab4735). Each lane contains 5µg of Drosophila embryo lysate.

      Lane 1: ab4735 1/500
      Lane 2: ab4735 1/500
      Lane 3: ab4735 1/1000
      Lane 4: ab4735 1/1000

      Expected molecular weight: 255kDa. We do not know the identity of the lower bands seen. 
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    实验方案

    文献

    This product has been referenced in:
    • Akpa MM  et al. Wilms Tumor Suppressor, WT1, Cooperates with MicroRNA-26a and MicroRNA-101 to Suppress Translation of the Polycomb Protein, EZH2, in Mesenchymal Stem Cells. J Biol Chem 291:3785-95 (2016). Read more (PubMed: 26655220) »
    • Jakob L  et al. Structural and functional insights into the fly microRNA biogenesis factor Loquacious. RNA 22:383-96 (2016). Read more (PubMed: 26769856) »

    See all 8 Publications for this product

    客户评价及客户问答

    Thank you for your enquiry. At Abcam we always interested in improving the quality of information on our datasheets. To this effect I would be very interested in determining the cross species reactivity of ab4735. Please can you tell me the application...

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