概述

  • 产品名称Anti-Dicer抗体[4A6]
    参阅全部 Dicer 一抗
  • 描述
    小鼠单克隆抗体[4A6] to Dicer
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to the central region of Dicer.

  • 阳性对照
    • Normal human ovary. Human placental tissue.

性能

应用

Our Abpromise guarantee covers the use of ab82539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 217 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

靶标

  • 功能Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
  • 疾病相关Pleuropulmonary blastoma
    Goiter multinodular 1, with or without Sertoli-Leydig cell tumors
    Rhabdomyosarcoma, embryonal, 2
    DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).
  • 序列相似性Belongs to the helicase family. Dicer subfamily.
    Contains 1 Dicer dsRNA-binding fold domain.
    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PAZ domain.
    Contains 2 RNase III domains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DCR antibody
    • DCR1 antibody
    • Dicer 1 ribonuclease type III antibody
    • Dicer antibody
    • DICER_HUMAN antibody
    • DICER1 antibody
    • Double-strand-specific ribonuclease antibody
    • Endoribonuclease Dicer antibody
    • Helicase MOI antibody
    • Helicase with RNase motif antibody
    • HERNA antibody
    • KIAA0928 antibody
    • MNG1 antibody
    • RMSE2 antibody
    see all

Anti-Dicer antibody [4A6] 图像

  • ab82539, at 1/20 dilution, staining Dicer in normal human ovary by Immunohistochemistry using paraffin-embedded tissue.


  • Predicted band size : 217 kDa
  • ab82539, at 1/20 dilution, staining Dicer in human lung tumor by Immunohistochemistry using paraffin-embedded tissue.

Anti-Dicer antibody [4A6] (ab82539)参考文献

This product has been referenced in:
  • Tan X  et al. A 5-microRNA signature for lung squamous cell carcinoma diagnosis and hsa-miR-31 for prognosis. Clin Cancer Res 17:6802-11 (2011). IHC-P ; Human . Read more (PubMed: 21890451) »

See 1 Publication for this product

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