Anti-Dicer抗体[4A6] (ab82539)
Key features and details
- Mouse monoclonal [4A6] to Dicer
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Dicer抗体[4A6]
参阅全部 Dicer 一抗 -
描述
小鼠单克隆抗体[4A6] to Dicer -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Normal human ovary. Human placental tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading...
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克隆
单克隆 -
克隆编号
4A6 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab82539于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/100 - 1/500. Predicted molecular weight: 217 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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说明 |
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WB
1/100 - 1/500. Predicted molecular weight: 217 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
靶标
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功能
Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes. -
疾病相关
Pleuropulmonary blastoma
Goiter multinodular 1, with or without Sertoli-Leydig cell tumors
Rhabdomyosarcoma, embryonal, 2
DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960). -
序列相似性
Belongs to the helicase family. Dicer subfamily.
Contains 1 Dicer dsRNA-binding fold domain.
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 PAZ domain.
Contains 2 RNase III domains. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 23405 Human
- Omim: 606241 Human
- SwissProt: Q9UPY3 Human
- Unigene: 738957 Human
- Unigene: 87889 Human
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别名
- DCR antibody
- DCR1 antibody
- Dicer 1 ribonuclease III antibody
see all
图片
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ab82539, at 1/20 dilution, staining Dicer in normal human ovary by Immunohistochemistry using paraffin-embedded tissue.
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ab82539 has been verified by siRNA target validation. The figure shows the elimination of the antigen band on western after treatment of cells with siRNA against human Dicer. No effect is seen on treatment with an irrelevant siRNA against GAPDH.
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ab82539, at 1/20 dilution, staining Dicer in human lung tumor by Immunohistochemistry using paraffin-embedded tissue.
数据表及文件
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Datasheet download
文献 (3)
ab82539 被引用在 3 文献中.
- Ohno SI et al. Nuclear microRNAs release paused Pol II via the DDX21-CDK9 complex. Cell Rep 39:110673 (2022). PubMed: 35417682
- Ohno S et al. Development of Novel Small Hairpin RNAs That do not Require Processing by Dicer or AGO2. Mol Ther 24:1278-89 (2016). PubMed: 27109632
- Tan X et al. A 5-microRNA signature for lung squamous cell carcinoma diagnosis and hsa-miR-31 for prognosis. Clin Cancer Res 17:6802-11 (2011). IHC-P ; Human . PubMed: 21890451