概述

  • 产品名称Anti-DIAPH1抗体
    参阅全部 DIAPH1 一抗
  • 描述
    兔多克隆抗体to DIAPH1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Pig, Orangutan
  • 免疫原

    Synthetic peptide, which represents a portion of the human DIAPH1 encoded within exon 1 (LocusLink ID 1729).

  • 阳性对照
    • Tested with 293T cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antibodies were affinity purified using the peptide immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/30000. Detects a band of approximately 200 kDa.

靶标

  • 功能Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
  • 组织特异性Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea.
  • 疾病相关Defects in DIAPH1 are the cause of deafness autosomal dominant type 1 (DFNA1) [MIM:124900]. DFNA1 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • 序列相似性Belongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • 结构域DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction (By similarity). DCAF7 binds to the FH2 (formin homology 2) domain.
  • 细胞定位Cell membrane. Cell projection > ruffle membrane. Cytoplasm > cytoskeleton. Membrane ruffles, especially at the tip of ruffles, of motile cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DIAPH1 antibody
    • deafness, autosomal dominant 1 antibody
    • DFNA1 antibody
    • DIA1 antibody
    • DIAP1 antibody
    • DIAP1_HUMAN antibody
    • DIAPH1 antibody
    • Diaphanous homolog 1 (Drosophila) antibody
    • diaphanous homolog 1 antibody
    • Diaphanous related formin 1 antibody
    • Diaphanous-related formin-1 antibody
    • DRF1 antibody
    • FLJ25265 antibody
    • hDIA1 antibody
    • LFHL1 antibody
    • low frequency hearing loss 1 antibody
    • p140DIA antibody
    • Protein diaphanous homolog 1 antibody
    see all

Anti-DIAPH1 antibody (ab11172)参考文献

This product has been referenced in:
  • Gulhan Ercan-Sencicek A  et al. Homozygous loss of DIAPH1 is a novel cause of microcephaly in humans. Eur J Hum Genet N/A:N/A (2014). Human . Read more (PubMed: 24781755) »
  • Xu Y  et al. Advanced glycation end product (AGE)-receptor for AGE (RAGE) signaling and up-regulation of Egr-1 in hypoxic macrophages. J Biol Chem 285:23233-40 (2010). WB ; Human . Read more (PubMed: 20507991) »

See all 2 Publications for this product

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