概述

  • 产品名称Anti-DHX8抗体
    参阅全部 DHX8 一抗
  • 描述
    小鼠单克隆抗体to DHX8
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: RREGRVANVA DVVSKGQRVK VKVLSFTGTK TSLSMKDVDQ ETGEDLNPNR RRNLVGETNE ETSMRNPDRP THLSLVSAPE VEDDSLERKR LTRISDPEKW , corresponding to amino acids 301-401 of Human DHX8

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab54592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.
  • 序列相似性Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 S1 motif domain.
  • 结构域The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent RNA helicase DHX8 antibody
    • ATP-dependent RNA helicase dhx8 antibody
    • DDX 8 antibody
    • DDX8 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 8 (RNA helicase) antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 8 antibody
    • DEAD/H box 8 antibody
    • DEAD/H box polypeptide 8 antibody
    • DEAH (Asp Glu Ala His) box polypeptide 8 antibody
    • DEAH box polypeptide 8 antibody
    • DEAH box protein 8 antibody
    • DHX 8 antibody
    • dhx8 antibody
    • DHX8_HUMAN antibody
    • HRH 1 antibody
    • HRH1 antibody
    • PRP 22 antibody
    • PRP22 antibody
    • PRP22, S. cerevisiae, homolog of antibody
    • PRPF 22 antibody
    • PRPF22 antibody
    • RNA helicase HRH 1 antibody
    • RNA helicase HRH1 antibody
    see all

Anti-DHX8 antibody 图像

  • DHX8 antibody (ab54592) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab54592 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54592, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-DHX8 antibody (ab54592)参考文献

ab54592 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab54592.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"