概述

  • 产品名称Anti-DHX57抗体
    参阅全部 DHX57 一抗
  • 描述
    兔多克隆抗体to DHX57
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 150 and 200 of human DHX57 (NP_945314.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab86784 was affinity purified using an epitope specific to DHX57 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab86784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 10 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 156 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Probable ATP-binding RNA helicase.
    • 序列相似性Belongs to the DEAD box helicase family. DEAH subfamily.
      Contains 1 C3H1-type zinc finger.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 UBA domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DDX57 antibody
      • DEAH (Asp Glu Ala Asp/His) box polypeptide 57 antibody
      • DEAH box protein 57 antibody
      • DEAH box RNA/DNA helicase AAM73547 antibody
      • DHX 57 antibody
      • DHX57 antibody
      • DHX57_HUMAN antibody
      • FLJ32861 antibody
      • Putative ATP dependent RNA helicase DHX57 antibody
      • Putative ATP-dependent RNA helicase DHX57 antibody
      see all

    Anti-DHX57 antibody 图像

    • All lanes : Anti-DHX57 antibody (ab86784) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 156 kDa
      Observed band size : 171 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • Detection of DHX57 by Western Blot of Immunprecipitate.
      ab86784, at 0.4 µg/ml, staining DHX57 in HeLa whole cell lysate immunoprecipitated using ab86784 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    Anti-DHX57 antibody (ab86784)参考文献

    ab86784 has not yet been referenced specifically in any publications.

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