概述

  • 产品名称Anti-DHX36抗体
    参阅全部 DHX36 一抗
  • 描述
    兔多克隆抗体to DHX36
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    A region corresponding to C terminal amino acids 950-1008 of Human DHX36 (NP_065916.1)

  • 阳性对照
    • HeLa whole cell lysate NIH3T3 whole cell lysate 293T whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/5000 - 1/15000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis.
  • 组织特异性Highly expressed in testis.
  • 序列相似性Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 细胞定位Nucleus. Cytoplasm. Isoform 1 preferentially localized to the nucleus and isoform 2 localized to the cytoplasm. However, partitioning of cellular localization between the nucleus and cytoplasm is not exclusive, as isoform 1 was also detected in the cytoplasm. Both isoforms were excluded from nucleoli.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DDX36 antibody
    • DEAH box protein 36 antibody
    • Dhx36 antibody
    • DHX36_HUMAN antibody
    • EC 3.6.1.- antibody
    • KIAA1488 antibody
    • MLE like protein 1 antibody
    • MLE-like protein 1 antibody
    • MLEL1 antibody
    • Probable ATP dependent RNA helicase DHX36 antibody
    • Probable ATP-dependent RNA helicase DHX36 antibody
    • RHAU antibody
    • RNA helicase associated with AU rich element ARE antibody
    • RNA helicase associated with AU-rich element ARE antibody
    see all

Anti-DHX36 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling DHX36 with ab70269 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DHX36 antibody (ab70269) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 115 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70269 at 3 µg/mg (lane 1) or control IgG (lane 2). 1/4 of the immunoprecipitate was loaded on to a gel, analysed by Western blot and revealed using ab70269 at 0.1 µg/ml.
  • ICC/IF image of ab70269 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70269, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab70269 staining in Human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70269, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-DHX36 antibody (ab70269)参考文献

This product has been referenced in:
  • Kralovicova J & Vorechovsky I Alternative splicing of U2AF1 reveals a shared repression mechanism for duplicated exons. Nucleic Acids Res N/A:N/A (2016). Read more (PubMed: 27566151) »
  • Bossa R  et al. Effect of various antitumor drugs on energetic processes. Pharmacol Res Commun 8:369-77 (1976). WB . Read more (PubMed: 68480) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (Ovary and testie)
Permeabilization Yes - PBST
Specification Ovary and testie
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
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提交于 May 02 2016

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (Primary aleveolar epithelial cells)
Specification Primary aleveolar epithelial cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

提交于 May 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"