概述

  • 产品名称Anti-DHX33抗体
    参阅全部 DHX33 一抗
  • 描述
    兔多克隆抗体to DHX33
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Rhesus monkey, Orangutan, Elephant
  • 免疫原

    A synthetic peptide corresponding to a region between residues 650 and the C terminus (residue 707) of human DHX33.

  • 阳性对照
    • Hela whole cell lysate; 293T cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab72451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
IP Use a concentration of 2 - 5 µg/ml.

靶标

  • 相关性DHX33 is a DEAD box protein whose function has not yet been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
  • 细胞定位Nucleus, nucleolus.
  • 数据库链接
  • 别名
    • DDX33 antibody
    • DEAD box polypeptide 33 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 33 antibody
    • DEAH (Asp Glu Ala His) box polypeptide 33 antibody
    • DEAH box protein 33 antibody
    • FLJ21972 antibody
    see all

Anti-DHX33 antibody 图像

  • All lanes : Anti-DHX33 antibody (ab72451) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 79 kDa
    Observed band size : 79 kDa
    Additional bands at : 200 kDa,31 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Immunoprecipitation of DHX33 from 1mg whole HeLa cells lysate by 3 ug ab72451 followed by WB (20% of IP loaded):
    Lane 1: ab72451 precipitate

    Lane 2: Control IgG

    For blotting immunoprecipitated DHX33, ab72451 was used at 1 ug/ml.

    Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-DHX33 antibody (ab72451)参考文献

ab72451 has not yet been referenced specifically in any publications.

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