Anti-DHX33抗体(ab72451)
Key features and details
- Rabbit polyclonal to DHX33
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DHX33抗体
参阅全部 DHX33 一抗 -
描述
兔多克隆抗体to DHX33 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Rhesus monkey, Orangutan, Elephant -
免疫原
Synthetic peptide within Human DHX33 aa 650 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_064547.2
Database link: Q9H6R0 -
阳性对照
- Hela whole cell lysate; 293T cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Affinity purified using an epitope specific to DHX33 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72451于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
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IP |
Use a concentration of 2 - 5 µg/ml.
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). |
IP
Use a concentration of 2 - 5 µg/ml. |
靶标
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相关性
DHX33 is a DEAD box protein whose function has not yet been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. -
细胞定位
Nucleus, nucleolus. -
数据库链接
- Entrez Gene: 56919 Human
- SwissProt: Q9H6R0 Human
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别名
- DDX33 antibody
- DEAD box polypeptide 33 antibody
- DEAD/H (Asp Glu Ala Asp/His) box polypeptide 33 antibody
see all
图片
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All lanes : Anti-DHX33 antibody (ab72451) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Observed band size: 79 kDa
Additional bands at: 200 kDa, 31 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunoprecipitation of DHX33 from 1mg whole HeLa cells lysate by 3 ug ab72451 followed by WB (20% of IP loaded):
Lane 1: ab72451 precipitate
Lane 2: Control IgG
For blotting immunoprecipitated DHX33, ab72451 was used at 1 ug/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab72451 尚未被引用在任何文献中。