The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
DHX33 is a DEAD box protein whose function has not yet been determined.
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling DHX33 with ab70863 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - DHX33 antibody (ab70863)
Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: ab70863 (Lane 1) was used for IP at 3 µg/mg lysate. DHX33 was also immunoprecipitated by rabbit anti-DHX33 antibody (lane 2) and rabbit IgG control (Lane 3). Detection: Chemiluminescence with an exposure time of 3 minutes.