概述

  • 产品名称Anti-DHX32抗体
    参阅全部 DHX32 一抗
  • 描述
    兔多克隆抗体to DHX32
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (EEEGLECPNS SSEKRYFPES LDSSDGDEEE VLACEDLELN PFDGLPYSSR) of human DHX32 (NP_060650).

  • 阳性对照
    • Human fetal lung lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab85459 is purified by a peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 组织特异性Expressed in lymphoid tissues (at protein level). Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung, lymphoid tissues and blood leukocytes.
  • 序列相似性Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 细胞定位Nucleus. Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3110079L04Rik antibody
    • AA408140 antibody
    • Ddx32 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 32 antibody
    • DEAD/H box 32 antibody
    • DEAD/H helicase like protein 1 antibody
    • DEAD/H helicase-like protein 1 antibody
    • DEAH (Asp-Glu-Ala-His) box polypeptide 32 antibody
    • DEAH box protein 32 antibody
    • DHLP1 antibody
    • DHX32 antibody
    • DHX32_HUMAN antibody
    • Helicase DDX32 antibody
    • HuDDX32 antibody
    • Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 antibody
    see all

Anti-DHX32 antibody 图像

  • ICC/IF image of ab85459 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85459 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-DHX32 antibody (ab85459) at 1 µg/ml + human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 84 kDa
    Observed band size : >90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

Anti-DHX32 antibody (ab85459)参考文献

ab85459 has not yet been referenced specifically in any publications.

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