概述

  • 产品名称Anti-DHX30抗体
    参阅全部 DHX30 一抗
  • 描述
    兔多克隆抗体to DHX30
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 1144 and 1194 of human DHX30 (NP_619520.1)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab85754 was affinity purified using an epitope specific to DHX30 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab85754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
IP Use at 5-10 µg/mg of lysate.

靶标

Anti-DHX30 antibody 图像

  • All lanes : Anti-DHX30 antibody (ab85754) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 134 kDa
    Observed band size : 134 kDa
    Additional bands at : 110 kDa,180 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of Human DHX3 by Western Blot of Immunprecipitate.
    All lanes: ab85754 at 0.4µg/ml staining DHX3 in HeLa whole cell lysates immunoprecipitated at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies:
    Lane 1: ab85754
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 3 minutes.

Anti-DHX30 antibody (ab85754)参考文献

ab85754 has not yet been referenced specifically in any publications.

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