概述

  • 产品名称Anti-DHX30抗体
    参阅全部 DHX30 一抗
  • 描述
    兔多克隆抗体to DHX30
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 350-400 of Human DHX30 (NP_619520.1)

  • 阳性对照
    • Hela cell lysate, 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab85687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 5 - 10µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 134 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-DHX30 antibody 图像

    • All lanes : Anti-DHX30 antibody (ab85687) at 0.04 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg


      Predicted band size : 134 kDa
      Observed band size : 134 kDa
      Additional bands at : 110 kDa,238 kDa,60 kDa. We are unsure as to the identity of these extra bands.
    • Detection of Human DHX30 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85687 at 10 ug/mg lysate. Subsequent Western blot analysis was perfomed using 0.4 ug/ml ab85687.

    Anti-DHX30 antibody (ab85687)参考文献

    ab85687 has not yet been referenced specifically in any publications.

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