Anti-DHX30抗体(ab85687)
Key features and details
- Rabbit polyclonal to DHX30
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DHX30抗体
参阅全部 DHX30 一抗 -
描述
兔多克隆抗体to DHX30 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide, corresponding to a region between amino acids 350-400 of Human DHX30 (NP_619520.1)
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阳性对照
- HeLa whole cell lysate (ab150035), 293T cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85687于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 134 kDa.
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IP |
Use at 5-10 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 134 kDa. |
IP
Use at 5-10 µg/mg of lysate. |
靶标
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序列相似性
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻译后修饰
Isoform 2 is phosphorylated on Ser-15. -
细胞定位
Cytoplasm and Mitochondrion. Cytoplasm. Mitochondrion matrix > mitochondrion nucleoid. - Information by UniProt
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数据库链接
- Entrez Gene: 22907 Human
- Entrez Gene: 72831 Mouse
- Entrez Gene: 367172 Rat
- GenBank: NM_138614 Human
- SwissProt: Q7L2E3 Human
- SwissProt: Q7L2E3-2 Human
- SwissProt: Q99PU8 Mouse
- SwissProt: Q5BJS0 Rat
see all -
别名
- DDX30 antibody
- DEAH box protein 30 antibody
- Dhx30 antibody
see all
图片
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All lanes : Anti-DHX30 antibody (ab85687) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 110 kDa, 238 kDa, 60 kDa. We are unsure as to the identity of these extra bands. -
Detection of Human DHX30 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85687 at 10 ug/mg lysate. Subsequent Western blot analysis was perfomed using 0.4 ug/ml ab85687.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab85687 被引用在 5 文献中.
- Ohkubo A et al. The FASTK family proteins fine-tune mitochondrial RNA processing. PLoS Genet 17:e1009873 (2021). PubMed: 34748562
- Chen G et al. A double-stranded RNA platform is required for the interaction between a host restriction factor and the NS1 protein of influenza A virus. Nucleic Acids Res 48:304-315 (2020). PubMed: 31754723
- Vassileff N et al. Revealing the Proteome of Motor Cortex Derived Extracellular Vesicles Isolated from Amyotrophic Lateral Sclerosis Human Postmortem Tissues. Cells 9:N/A (2020). PubMed: 32708779
- Murat P et al. RNA G-quadruplexes at upstream open reading frames cause DHX36- and DHX9-dependent translation of human mRNAs. Genome Biol 19:229 (2018). PubMed: 30591072
- Antonicka H et al. A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability. EMBO Rep 18:28-38 (2017). PubMed: 27974379