概述

  • 产品名称
  • 描述
    兔多克隆抗体to DHX29
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Dog, Chimpanzee, Gorilla, Chinese hamster
  • 免疫原

    Synthetic peptide corresponding to a region between residue 150 and 200 of Human DHX29 (NP_061903.2).

  • 阳性对照
    • HeLa and 293T cell lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2500. Detects a band of approximately 175 kDa (predicted molecular weight: 155 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性
    DHX29 belongs to the DEAD box helicase family in the DEAH subfamily. It contains 1 helicase ATP-binding domain and 1 helicase C-terminal domain. DHX29 may be involved in ATP-dependent DNA or RNA unwinding.
  • 细胞定位
    Cytoplasmic (Probable)
  • 数据库链接
  • 别名
    • DDX29 antibody
    • DEAH (Asp Glu Ala Asp/His) box polypeptide 29 antibody
    • DEAH (Asp Glu Ala His) box polypeptide 29 antibody
    • DEAH box protein 29 antibody
    • Nucleic acid helicase DDXx antibody
    • Putative ATP dependent RNA helicase DHX29 antibody
    see all

Anti-DHX29 antibody 图像

  • All lanes : Anti-DHX29 antibody (ab70745) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 155 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • 1mg whole cell lysate fro HeLa cells was immunoprecipitated using ab70745 at 3µg/mg lysate. For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70745 at 1ug/ml. Detection: chemiluminescence with exposure time of 3 minutes.

Anti-DHX29 antibody (ab70745)参考文献

ab70745 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (U2OS cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
U2OS cells
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Abcam user community

Verified customer

提交于 Jul 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS Cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
20 µg
Specification
U2OS Cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

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Verified customer

提交于 Jul 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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