概述

  • 产品名称Anti-DHX15抗体
    参阅全部 DHX15 一抗
  • 描述
    兔多克隆抗体to DHX15
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between C terminal residues 750-795 of human DHX15 (Swiss-Prot O43143).

  • 阳性对照
    • 293T whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab70454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/15000. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa).
IP Use at 1-5 µg/mg of lysate.

靶标

Anti-DHX15 antibody 图像

  • All lanes : Anti-DHX15 antibody (ab70454) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from 293T cells.
    Lane 2 : Whole cell lysate from 293T cells.

    Lysates/proteins at 60 µg per lane.


    Predicted band size : 91 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
  • 1 mg 293T whole cell lysate. ab70454 used at 0.1 µg/ml for the Western blot and at 1 µg/mg lysate for Immunoprecipitation.

Anti-DHX15 antibody (ab70454)参考文献

This product has been referenced in:
  • Chen YL  et al. The telomerase inhibitor Gno1p/PINX1 activates the helicase Prp43p during ribosome biogenesis. Nucleic Acids Res 42:7330-45 (2014). WB ; Human . Read more (PubMed: 24823796) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"