Anti-DHRS7抗体(ab69348)

概述

  • 产品名称Anti-DHRS7抗体
    参阅全部 DHRS7 一抗
  • 描述
    小鼠多克隆抗体to DHRS7
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length DHRS7 protein (Human) (NP_057113.1)

  • 阳性对照
    • DHRS7 transfected 293T cell lysate and human kidney tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab69348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明WB: 1/500 - 1/1000. Detects a band of approximately 36 and 37 kDa (predicted molecular weight: 38 kDa).
    Detection Antibody.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 序列相似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CGI 86 antibody
      • CGI-86 antibody
      • CGI86 antibody
      • Dehydrogenase/reductase (SDR family) member 7 antibody
      • Dehydrogenase/reductase SDR family member 7 antibody
      • Dhrs7 antibody
      • DHRS7_HUMAN antibody
      • DHRS7A antibody
      • retDSR4 antibody
      • Retinal short chain dehydrogenase/reductase 4 antibody
      • Retinal short-chain dehydrogenase/reductase 4 antibody
      • retSDR4 antibody
      • SDR34C1 antibody
      • Short chain dehydrogenase/reductase family 34C, member 1 antibody
      see all

    Anti-DHRS7 antibody 图像

    • Anti-DHRS7 antibody (ab69348) at 1/500 dilution + human kidney tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 38 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-DHRS7 antibody (ab69348) at 1/500 dilution

      Lane 1 : DHRS7 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 38 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • IHC-P image of DHRS7 (ab69348) on Human Pancreas sections. The sections were fixed in paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in H2O2 solution for 10 mins at 22°C.

      See Abreview

    Anti-DHRS7 antibody (ab69348)参考文献

    ab69348 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Pancreas)
    Specification Pancreas
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
    Permeabilization No
    Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3µg/mL · Temperature: 22°C
    Username

    Dr. Aamir Ahmed

    Verified customer

    提交于 Sep 23 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"