概述

  • 产品名称Anti-DHRS2抗体
    参阅全部 DHRS2 一抗
  • 描述
    兔多克隆抗体to DHRS2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cat, Dog
  • 免疫原

    Synthetic peptide desgined within residues: LEHCGGVDFL VCSAGVNPLV GSTLGTSEQI WDKILSVNVK SPALLLSQLL, corresponding to internal sequence amino acids 108-158 of Human DHRS2 (NP_005785)

  • 阳性对照
    • 721_B cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

靶标

  • 相关性DHRS2 belongs to the short-chain dehydrogenases/reductases (SDR) family. It may inhibit cell replication either by catalyzing the oxidation of estrogen and androgen or by converting cortisone in cortisol.
  • 细胞定位Mitochondrion matrix. Nucleus. Note: A minor fraction of the protein is translocated from the mitochondria to the nucleus, after cleavage of the targeting signal.
  • 数据库链接
  • 别名
    • 5430405K24Rik antibody
    • Dehydrogenase/reductase (SDR family) member 2 antibody
    • Dehydrogenase/reductase member 2 antibody
    • Dehydrogenase/reductase SDR family member 2 antibody
    • Dehydrogenase/reductase SDR family member 2 mitochondria antibody
    • Dicarbonyl reductase HEP27 antibody
    • Hep27 antibody
    • HEP27 protein antibody
    • MGC143467 antibody
    • Protein D antibody
    • SDR family member antibody
    • SDR25C1 antibody
    • Short chain alcohol dehydrogenase family member antibody
    • Short chain dehydrogenase/reductase family 25C member 1 antibody
    see all

Anti-DHRS2 antibody 图像

  • Anti-DHRS2 antibody (ab83254) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

Anti-DHRS2 antibody (ab83254)参考文献

ab83254 has not yet been referenced specifically in any publications.

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