The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
功能Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.
通路Protein modification; eIF5A hypusination.
序列相似性Belongs to the deoxyhypusine synthase family.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.