概述

  • 产品名称
    DHEA sulfate (DHEA-S) ELISA试剂盒
    参阅全部 DHEA-S 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Control 64 < 7.9%
    批次间
    样品 n Mean SD CV%
    Control 48 < 10.4%
  • 样品类型
    Serum, Plasma
  • 检测类型
    Competitive
  • 灵敏度
    = 0.04 ng/ml
  • 范围
    0.1 µg/ml - 10 µg/ml
  • 复检

    = 96.71 %

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s DHEA sulfate (DHEA-S) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of DHEA sulfate in plasma and serum.

     

    A 96-well plate has been precoated with anti-DHEA sulphate antibodies. Samples and the DHEA sulfate-HRP conjugate are added to the wells, where DHEA sulfate in the sample competes with the added DHEA sulfate-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of DHEA sulfate in the sample and the intensity is measured at 450 nm.

  • 经测试应用
    适用于: Competitive ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    10X Wash Solution 1 x 50ml
    Serum Diluent 1 x 100ml
    Anti-DHEA sulfate antibody Coated Microplate (12 x 8 wells) 1 unit
    Control A 1 x 1ml
    Control B 1 x 1ml
    Cover foils 1 unit
    DHEA sulfate Standard 0 – 0 µg/mL 1 x 1ml
    DHEA sulfate Standard 1 – 0.1 µg/mL 1 x 1ml
    DHEA sulfate Standard 2 – 0.4 µg/mL 1 x 1ml
    DHEA sulfate Standard 3 – 1.0 µg/mL 1 x 1ml
    DHEA sulfate Standard 4 – 4.0 µg/mL 1 x 1ml
    DHEA sulfate Standard 5 – 10.0 µg/mL 1 x 1ml
    DHEA sulfate-HRP Conjugate 1 x 12ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab108669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent dilution.

图片

  • Representative Standard Curve using ab108669

实验方案

文献

This product has been referenced in:
  • Grimby-Ekman A  et al. Different DHEA-S Levels and Response Patterns in Individuals with Chronic Neck Pain, Compared with a Pain Free Group-a Pilot Study. Pain Med N/A:N/A (2016). Competitive ELISA ; Human . Read more (PubMed: 27498549) »

See 1 Publication for this product

客户评价及客户问答

The standard range of 0.1 - 10 ug/ml takes into account the 1/50 sample dilution that is done, so you do not need to multiply your end measurement by 50.

DHEA-S and DHEA are very similar molecules: our antibody has more affinity for DHEA compared to DHEA-S (10 % higher). You have to consider that in human blood the quantity of DHEA is 1.000 times lower than DHEA-S (from clinical point of view the elevat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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