DHEA sulfate (DHEA-S) ELISA试剂盒(ab108669)


  • 产品名称DHEA sulfate (DHEA-S) ELISA试剂盒
    参阅全部 DHEA-S 试剂盒
  • 检测方法Colorimetric
  • 精确度
    样品 n Mean SD CV%
    Control 64 < 5.7%
    样品 n Mean SD CV%
    Control 48 < 9.6%
  • 测试
    1 x 96 well plate
  • 样品类型
    Serum, Plasma
  • 检测类型Competitive
  • 灵敏度
    = 0.03 ng/ml
  • 范围
    0.1 µg/ml - 10 µg/ml
  • 复检

    = 96.71 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s DHEA sulfate (DHEA-S) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of DHEA sulfate in plasma and serum.


    A 96-well plate has been precoated with anti-DHEA sulphate antibodies. Samples and the DHEA sulfate-HRP conjugate are added to the wells, where DHEA sulfate in the sample competes with the added DHEA sulfate-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of DHEA sulfate in the sample and the intensity is measured at 450 nm.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate


  • 存放说明Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    5X Serum Diluent 1 x 20ml
    Anti-DHEA sulfate IgG Coated Microplate (12 x 8 wells) 1 unit
    Cover foils 1 unit
    DHEA sulfate control 1 x 1ml
    DHEA sulfate Standard 0 – 0 µg/mL 1 x 1ml
    DHEA sulfate Standard 1 – 0.1 µg/mL 1 x 1ml
    DHEA sulfate Standard 2 – 0.4 µg/mL 1 x 1ml
    DHEA sulfate Standard 3 – 1.0 µg/mL 1 x 1ml
    DHEA sulfate Standard 4 – 4.0 µg/mL 1 x 1ml
    DHEA sulfate Standard 5 – 10.0 µg/mL 1 x 1ml
    DHEA sulfate-HRP Conjugate 1 x 12ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • 研究领域


    Our Abpromise guarantee covers the use of ab108669 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Competitive ELISA Use at an assay dependent dilution.

    DHEA sulfate (DHEA-S) ELISA Kit 图像

    • Representative Standard Curve using ab108669


    DHEA sulfate (DHEA-S) ELISA Kit (ab108669)参考文献

    ab108669 has not yet been referenced specifically in any publications.

    Product Wall

    The standard range of 0.1 - 10 ug/ml takes into account the 1/50 sample dilution that is done, so you do not need to multiply your end measurement by 50.

    DHEA-S and DHEA are very similar molecules: our antibody has more affinity for DHEA compared to DHEA-S (10 % higher). You have to consider that in human blood the quantity of DHEA is 1.000 times lower than DHEA-S (from clinical point of view the elevat...

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