Anti-DHEA 3-Sulfate抗体(ab36365)

概述

  • 产品名称
    Anti-DHEA 3-Sulfate抗体
    参阅全部 DHEA 3-Sulfate 一抗
  • 描述
    羊多克隆抗体to DHEA 3-Sulfate
  • 特异性
    Cross reactivity : DHEAS 100% DHEA 69% 5-alpha-Androstendione 5.3% 5 Androstendione 3.6% 4 Androstendione 2.3% Androstendione 3.0% Testosterone <0.3% Androstendiol <0.4%
  • 经测试应用
    适用于: RIAmore details
  • 免疫原

    DHEA 3-Sulfate conjugated to HMS-BSA

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    Blue Sepharose chromatography.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab36365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
RIA
  • 应用说明
    RIA: 100 µl/test (3H).

    Assay buffer : 0.01 M PBS, pH 7.4 with 0.1% gelatin and 0.1% thiomersal.

    Add 500 µl buffer and divide into 100 µl aliquots for storage at -20ºC. When required, dilute each aliquot to 10 ml using assay buffer. Use 100 µl/test.

    Use Dextran coated charcoal to separate.

    The antiserum is sufficiently specific and does not require pre-assay chromatographic purification. No extraction of the sample is required.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      DHEA (Dehydroepiandrosterone) is an endogenous steroid hormone made in the human body, and secreted by the adrenal gland. DHEA serves as precursor to male and female sex hormones (androgens and estrogens). Dehydroepiandrosterone sulfate (DHEAS) is the sulfated version of DHEA. This conversion is reversibly catalysed by sulfotransferase (SULT2A1) primarily in the adrenals, the liver, and small intestines. In blood, most DHEA is found as DHEAS with levels that are about 300 times higher than free DHEA. While DHEA levels reach their peak in the early morning hours, DHEAS levels show no diurnal variation. DHEAS is biologically active only after its sulfate group has been split and it becomes DHEA again. Measurement of DHEAS is preferable to DHEA as levels are more stable.
    • 细胞定位
      Secreted
    • 别名
      • Dehydroepiandrosterone sulfate antibody

    文献

    ab36365 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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