Anti-DHCR7抗体- C-terminal (ab226784)
Key features and details
- Rabbit polyclonal to DHCR7 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-DHCR7抗体- C-terminal
参阅全部 DHCR7 一抗 -
描述
兔多克隆抗体to DHCR7 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow -
免疫原
Recombinant fragment within Human DHCR7 (C terminal). The exact sequence is proprietary.
Database link: Q9UBM7 -
阳性对照
- IHC-P: Mouse liver and rat duodenum tissues. WB: HepG2 whole cell and membrane extracts.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226784于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
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说明 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. |
靶标
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相关性
The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[OMIM] -
细胞定位
Endoplasmic reticulum membrane; Multi-pass membrane protein -
数据库链接
- Entrez Gene: 514745 Cow
- Entrez Gene: 1717 Human
- Entrez Gene: 13360 Mouse
- Entrez Gene: 64191 Rat
- Omim: 602858 Human
- SwissProt: Q5E9J5 Cow
- SwissProt: Q9UBM7 Human
- SwissProt: O88455 Mouse
see all -
别名
- 7 dehydrocholesterol reductase antibody
- 7 DHC reductase antibody
- Delta 7 dehydrocholesterol reductase antibody
see all
图片
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All lanes : Anti-DHCR7 antibody - C-terminal (ab226784) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract, boiled
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) membrane extract, boiled
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract, unboiled
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) membrane extract, unboiled
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded mouse liver tissue stained for DHCR7 using ab226784 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded rat duodenum tissue stained for DHCR7 using ab226784 at 1/500 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226784 尚未被引用在任何文献中。