概述

  • 产品名称Anti-DGKH抗体
    参阅全部 DGKH 一抗
  • 描述
    兔多克隆抗体to DGKH
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • 免疫原

    A synthetic peptide corresponding to a region of Human DGKH

  • 阳性对照
    • Fetal heart lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab80693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/1562500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).Can be blocked with Human DGKH peptide (ab99354). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/500.

靶标

  • 相关性This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family of proteins, specifically the type II DGK subfamily. Members of this family are involved in regulating the intracellular concentrations of diacylglycerol and phosphatidic acid. Two transcript variants encoding distinct isoforms have been identified for this gene.
  • 细胞定位Cytoplasm. Translocated from the cytoplasm to endosomes in response to stress stimuli. Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association.
  • 数据库链接
  • 别名
    • DAG kinase eta antibody
    • DGK eta antibody
    • Diacylglycerol kinase eta antibody
    • Diglyceride kinase eta antibody

Anti-DGKH antibody 图像

  • Sample Type : Adult mouse cortex. Primary Antibody Dilution : 1:500. Secondary Antibody : Anti-rabbit-Cy3. Secondary Antibody Dilution : 1:1000. Color/Signal Descriptions : Red: DGKH Cyan: Nissl (Neurons). Gene Name : DGKH.
  • Anti-DGKH antibody (ab80693) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 134 kDa
    Observed band size : 134 kDa
    Gel concentration 6-18%

Anti-DGKH antibody (ab80693)参考文献

This product has been referenced in:
  • Martinez K  et al. Diacylglycerol kinase inhibitor R59022 attenuates conjugated linoleic acid-mediated inflammation in human adipocytes. J Lipid Res 54:662-70 (2013). Human . Read more (PubMed: 23264678) »

See 1 Publication for this product

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