概述

  • 产品名称Anti-DGKA抗体
    参阅全部 DGKA 一抗
  • 描述
    兔多克隆抗体to DGKA
  • 特异性This antibody detects endogenous levels of total DGKA protein.
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to internal sequence amino acids 331-370 of Human DGKA

  • 阳性对照
    • Extracts from Jurkat cells. IHC-P (FFPE): Human Skin (Normal) tissue

性能

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应用

Our Abpromise guarantee covers the use of ab64845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ELISA 1/20000.
IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.
  • 组织特异性Lymphocytes and oligodendroglial cells.
  • 序列相似性Belongs to the eukaryotic diacylglycerol kinase family.
    Contains 1 DAGKc domain.
    Contains 2 EF-hand domains.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 80 kDa diacylglycerol kinase antibody
    • DAG kinase alpha antibody
    • DAGK antibody
    • DAGK1 antibody
    • DGK alpha antibody
    • DGK-alpha antibody
    • dgkA antibody
    • DGKA_HUMAN antibody
    • Diacylglycerol kinase alpha antibody
    • Diacylglycerol kinase, alpha 80kDa antibody
    • Diglyceride kinase alpha antibody
    • MGC12821 antibody
    • MGC42356 antibody
    • OTTHUMP00000242836 antibody
    • OTTHUMP00000242955 antibody
    • OTTHUMP00000244046 antibody
    see all

Anti-DGKA antibody 图像

  • IHC image of DGKA staining in Human Skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64845, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • All lanes : Anti-DGKA antibody (ab64845) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with blocking peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • ICC/IF image of ab64845 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64845, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DGKA antibody (ab64845)参考文献

ab64845 has not yet been referenced specifically in any publications.

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