Anti-DGKA抗体[8G5] (ab119261)

概述

  • 产品名称Anti-DGKA抗体[8G5]
    参阅全部 DGKA 一抗
  • 描述
    小鼠单克隆抗体[8G5] to DGKA
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Dog, Human, Monkey
  • 免疫原

    Recombinant full length Human DGKA produced in HEK293T cells (NP_001336).

  • 阳性对照
    • HEK293T cell lysate transfected with pCMV6-ENTRY DGKA cDNA; HeLa, COS7, Jurkat and MDCK cell extracts; COS7 cells transiently transfected by pCMV6-ENTRY DGKA.
  • 常规说明Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

性能

应用

Our Abpromise guarantee covers the use of ab119261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 83 kDa.
ICC/IF 1/100.

靶标

  • 功能Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.
  • 组织特异性Lymphocytes and oligodendroglial cells.
  • 序列相似性Belongs to the eukaryotic diacylglycerol kinase family.
    Contains 1 DAGKc domain.
    Contains 2 EF-hand domains.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 80 kDa diacylglycerol kinase antibody
    • DAG kinase alpha antibody
    • DAGK antibody
    • DAGK1 antibody
    • DGK alpha antibody
    • DGK-alpha antibody
    • dgkA antibody
    • DGKA_HUMAN antibody
    • Diacylglycerol kinase alpha antibody
    • Diacylglycerol kinase, alpha 80kDa antibody
    • Diglyceride kinase alpha antibody
    • MGC12821 antibody
    • MGC42356 antibody
    • OTTHUMP00000242836 antibody
    • OTTHUMP00000242955 antibody
    • OTTHUMP00000244046 antibody
    see all

Anti-DGKA antibody [8G5] 图像

  • All lanes : Anti-DGKA antibody [8G5] (ab119261) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY DGKA cDNA

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique

    Predicted band size : 83 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC222395)
  • All lanes : Anti-DGKA antibody [8G5] (ab119261) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : Extract from COS7 cell line
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 83 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC222395)
  • ab119261 at 1/100 dilution staining DGKA in COS7 cells transiently transfected with pCMV6-ENTRY DGKA by Immunofluorescence.

Anti-DGKA antibody [8G5] (ab119261)参考文献

ab119261 has not yet been referenced specifically in any publications.

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