Anti-DGAT2抗体(ab59493)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to DGAT2
  • 特异性
    This antibody is not expected to cross-react with other DGAT members.
  • 经测试应用
    适用于: Sandwich ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-KLEHPTQQDIDLYH

    , corresponding to C terminal amino acids 346-359 of Human DGAT2

性能

应用

Our Abpromise guarantee covers the use of ab59493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml.

靶标

  • 功能
    Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation.
  • 组织特异性
    Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin.
  • 通路
    Glycerolipid metabolism; triacylglycerol biosynthesis.
  • 序列相似性
    Belongs to the diacylglycerol acyltransferase family.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DGAT2 antibody
    • DGAT2_HUMAN antibody
    • Diacylglycerol O acyltransferase like protein 2 antibody
    • Diacylglycerol O-acyltransferase 2 antibody
    • Diacylglycerol O-acyltransferase homolog 2 (mouse) antibody
    • Diacylglycerol O-acyltransferase homolog 2 antibody
    • Diacylglycerol O-acyltransferase-like protein 2 isoform 1 antibody
    • Diglyceride acyltransferase 2 antibody
    • DKFZp686A15125 antibody
    • GS1999full antibody
    • HMFN1045 antibody
    see all

图片

  • ab59493 at 3ug/ml staining DGAT2 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval by microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.

  • All lanes : Anti-DGAT2 antibody (ab59493) at 0.3 µg/ml

    Lane 1 : Cell lysates prepared from DGAT2 transiently transfected HEK293 cells.
    Lane 2 : Cell lysates prepared from mock transfected HEK293 cells.
    Lane 3 : Tissue lysates prepared from human liver tissue.


    Observed band size : 45,60 kDa (why is the actual band size different from the predicted?)
  • Anti-DGAT2 antibody (ab59493) at 0.5 µg/ml + Human Liver lysate at 35 µg

文献

ab59493 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Total protein in input
500 µg
Specification
Hepa1-6 hepatoma cells
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

提交于 Sep 05 2012

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Loading amount
40 µg
Specification
Hepa1-6 hepatoma cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 17 2012

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse muscle)
Loading amount
40 µg
Specification
Mouse muscle
Gel Running Conditions
Non-reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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