Anti-DGAT2抗体(ab59493)

概述

  • 产品名称Anti-DGAT2抗体
    参阅全部 DGAT2 一抗
  • 描述
    山羊多克隆抗体to DGAT2
  • 特异性This antibody is not expected to cross-react with other DGAT members.
  • 经测试应用适用于: Sandwich ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-KLEHPTQQDIDLYH

    , corresponding to C terminal amino acids 346-359 of Human DGAT2

性能

应用

Our Abpromise guarantee covers the use of ab59493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml.

靶标

  • 功能Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation.
  • 组织特异性Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin.
  • 通路Glycerolipid metabolism; triacylglycerol biosynthesis.
  • 序列相似性Belongs to the diacylglycerol acyltransferase family.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DGAT2 antibody
    • DGAT2_HUMAN antibody
    • Diacylglycerol O acyltransferase like protein 2 antibody
    • Diacylglycerol O-acyltransferase 2 antibody
    • Diacylglycerol O-acyltransferase homolog 2 (mouse) antibody
    • Diacylglycerol O-acyltransferase homolog 2 antibody
    • Diacylglycerol O-acyltransferase-like protein 2 isoform 1 antibody
    • Diglyceride acyltransferase 2 antibody
    • DKFZp686A15125 antibody
    • GS1999full antibody
    • HMFN1045 antibody
    see all

Anti-DGAT2 antibody 图像

  • ab59493 at 3ug/ml staining DGAT2 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval by microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.

  • All lanes : Anti-DGAT2 antibody (ab59493) at 0.3 µg/ml

    Lane 1 : Cell lysates prepared from DGAT2 transiently transfected HEK293 cells.
    Lane 2 : Cell lysates prepared from mock transfected HEK293 cells.
    Lane 3 : Tissue lysates prepared from human liver tissue.


    Observed band size : 45,60 kDa (why is the actual band size different from the predicted?)
  • Anti-DGAT2 antibody (ab59493) at 0.5 µg/ml + Human Liver lysate at 35 µg

Anti-DGAT2 antibody (ab59493)参考文献

ab59493 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Total protein in input 500 µg
Specification Hepa1-6 hepatoma cells
Immuno-precipitation step Protein A
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提交于 Sep 05 2012

Application Western blot
Sample Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Loading amount 40 µg
Specification Hepa1-6 hepatoma cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Apr 17 2012

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse muscle)
Loading amount 40 µg
Specification Mouse muscle
Gel Running Conditions Non-reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Nov 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"