概述

  • 产品名称Anti-Dexras1抗体
    参阅全部 Dexras1 一抗
  • 描述
    兔多克隆抗体to Dexras1
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Human
  • 免疫原

    Synthetic peptide

  • 阳性对照
    • Mouse brain extract This antibody gave a positive result in IHC in the following FFPE tissue: Mouse kidney.

性能

应用

Our Abpromise guarantee covers the use of ab78459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 32 kDa.
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Small GTPase. Negatively regulates the transcription regulation activity of the APBB1/FE65-APP complex via its interaction with APBB1/FE65.
  • 组织特异性Expressed in a variety of tissues including heart, cardiovascular tissues, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, gastrointestinal and reproductive tissues.
  • 序列相似性Belongs to the small GTPase superfamily. RasD family.
  • 翻译后修饰S-nitrosylation stimulates guanine-nucleotide exchange activity.
  • 细胞定位Cell membrane. Cytoplasm > perinuclear region. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activator of G protein signaling 1 antibody
    • Activator of G protein signaling antibody
    • Activator of G-protein signaling 1 antibody
    • AGS 1 antibody
    • AGS1 antibody
    • Dexamethasone induced ras related protein 1 antibody
    • Dexamethasone-induced ras protein 1 antibody
    • Dexamethasone-induced Ras-related protein 1 antibody
    • Dexras 1 antibody
    • MGC:26290 antibody
    • RAS dexamethasone induced 1 antibody
    • Ras protein, Dexamethasone-induced, 1 antibody
    • Ras related protein antibody
    • RASD 1 antibody
    • Rasd1 antibody
    • RASD1_HUMAN antibody
    see all

Anti-Dexras1 antibody 图像

  • Anti-Dexras1 antibody (ab78459) at 1/1000 dilution + mouse brain extract

    Predicted band size : 32 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab78459 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78459, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of Dexras1 staining in Mouse kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78459, 1µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Dexras1 antibody (ab78459)参考文献

This product has been referenced in:
  • Lellis-Santos C  et al. The regulation of Rasd1 expression by glucocorticoids and prolactin controls peripartum maternal insulin secretion. Endocrinology 153:3668-78 (2012). Read more (PubMed: 22700767) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab78459.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"