Anti-Detyrosinated + Decarboxylated alpha Tubulin抗体[AA12] (ab24622)


  • 产品名称Anti-Detyrosinated + Decarboxylated alpha Tubulin抗体[AA12]
  • 描述
    小鼠单克隆抗体[AA12] to Detyrosinated + Decarboxylated alpha Tubulin
  • 特异性ab24622 recognises detyrosinated and decarboxylated alpha Tubulin.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rabbit
    预测可用于: Mammal
  • 免疫原

    This antibody was raised against the peptide sequence




  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.03% Thimerosal (merthiolate)
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号AA12
  • 同种型IgG2a
  • 研究领域


Our Abpromise guarantee covers the use of ab24622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21421796
WB 1/1000. Predicted molecular weight: 50 kDa.


  • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 序列相似性Belongs to the tubulin family.
  • 翻译后修饰Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 细胞定位Cytoplasm, cytoskeleton.
  • Information by UniProt
  • 别名
    • Alpha-tubulin 1 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • Tuba4a antibody
    • Tubulin alpha-1 chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    see all

Anti-Detyrosinated + Decarboxylated alpha Tubulin antibody [AA12] (ab24622)参考文献

This product has been referenced in:
  • Nagai J  et al. Crmp4 deletion promotes recovery from spinal cord injury by neuroprotection and limited scar formation. Sci Rep 5:8269 (2015). IHC-Fr ; Mouse . Read more (PubMed: 25652774) »
  • Akisaka T  et al. Differential distribution of posttranslationally modified microtubules in osteoclasts. J Histochem Cytochem 59:630-8 (2011). ICC/IF ; Rabbit . Read more (PubMed: 21421796) »

See all 3 Publications for this product

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My apologies for the duplicate email, but I forgot to mention that I have added this code to your review and it is now active and ready to be used.

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Thank you very much for your email, and for your review of ab24622.

This code expired in August, which is probably the reason why it is not working at this time. Although we normally don't issue new codes past the expiration date, I've gone a...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (E18 primary hippocampal neurons)
Specification E18 primary hippocampal neurons
Fixative PHEM buffer (60 mM Pipes, 25 mM Hepes, 5 mM EGTA, and 1 mM MgCl) containing 0.25% glutaraldehyde, 3.7% paraformaldehyde, 3.7% sucrose, and 0.1% Triton X-100
Permeabilization Yes - Triton X-100 (in fixation solution)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

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Verified customer

提交于 Jun 20 2012

Thank you for your reply. I have issued the discount code below, and please let me know if you have any questions or if there is anything else that we can do for you.

Expiration date: August 18, 2012

This cod...

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Thank you for contacting us with your enquiry.

To our knowledge, ab24622 has not been tested in rat samples or in IP.So this antibody will quality for our testing discount program. In this program,youwould initally purchaseab24622, test it i...

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Expiration date: XX-XXX-XX

I am very pleased to hear you would like to accept our offer and test ab24622 in rat species with ICC/IF. This code will give you 1 free primary antibody before the expiration date. T...

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Thank you for contacting us.

This antibody has not been tested in rat species, but it’s very likely it will cross react with this species, due to sequence homology.

If you are interested in testing ab24622 in rat samples with ...

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