Anti-Desmoplakin I+II抗体(ab118804)

概述

  • 产品名称Anti-Desmoplakin I+II抗体
    参阅全部 Desmoplakin I+II 一抗
  • 描述
    兔多克隆抗体to Desmoplakin I+II
  • 经测试应用适用于: IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 1900-1950 of Human Desmoplakin I+II (NP_004406.2).

  • 阳性对照
    • HeLa whole cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab118804 was affinity purified using an epitope specific to Desmoplakin I+II immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab118804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-10 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.
    • 组织特异性Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin.
    • 疾病相关Keratoderma, palmoplantar, striate 2
      Cardiomyopathy, dilated, with woolly hair and keratoderma
      Arrhythmogenic right ventricular dysplasia, familial, 8
      Skin fragility-woolly hair syndrome
      Epidermolysis bullosa, lethal acantholytic
      Cardiomyopathy, dilated, with woolly hair, keratoderma, and tooth agenesis
    • 序列相似性Belongs to the plakin or cytolinker family.
      Contains 17 plectin repeats.
      Contains 1 SH3 domain.
      Contains 6 spectrin repeats.
    • 结构域Its association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins.
      The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1.
      The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments.
    • 翻译后修饰Ser-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments.
      Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (PubMed:9651377).
    • 细胞定位Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane. Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network (PubMed:12802069). Localizes at the intercalated disk in cardiomyocytes (By similarity).
    • Information by UniProt
    • 数据库链接
    • 别名
      • 250/210 kDa paraneoplastic pemphigus antigen antibody
      • DCWHKTA antibody
      • Desmoplakin (DPI DPII) antibody
      • Desmoplakin antibody
      • Desmoplakin I antibody
      • Desmoplakin II antibody
      • DESP_HUMAN antibody
      • DP antibody
      • DP I antibody
      • DP II antibody
      • DPI antibody
      • DPII antibody
      • DSP antibody
      • KPPS2 antibody
      • PPKS 2 antibody
      • PPKS2 antibody
      see all

    Anti-Desmoplakin I+II antibody 图像

    • Detection of Desmoplakin I+II in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118804 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-Desmoplakin I+II antibody which recognizes a downstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-Desmoplakin I+II antibody (ab118804)参考文献

    ab118804 has not yet been referenced specifically in any publications.

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