Anti-Desmoplakin I+II抗体[DP2.15] (ab16434)
Key features and details
- Mouse monoclonal [DP2.15] to Desmoplakin I+II
- Suitable for: WB, ICC, ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat, Chicken, Cow, Human
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Desmoplakin I+II抗体[DP2.15]
参阅全部 Desmoplakin I+II 一抗 -
描述
小鼠单克隆抗体[DP2.15] to Desmoplakin I+II -
宿主
Mouse -
经测试应用
适用于: WB, ICC, ICC/IF, IHC-Frmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Chicken, Cow, Human -
免疫原
Full length protein (Cow).
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阳性对照
- Positive cell lines: Human A432 and MCF-7; Rat MH1C1; Bovine MDBK and BMGE. Stratified epithelia, simple epithelia, including glands, urothelium, thymic reticular epithelium, hepatocytes, intercalated disks of myocardium and arachnoid cells of meninges.
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常规说明
Suitable for use in the detection of primary and metastatic carcinomas.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: PBS, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
单克隆 -
克隆编号
DP2.15 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16434于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
Use at an assay dependent concentration.
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ICC | (1) |
Use at an assay dependent concentration.
|
ICC/IF | (1) |
Use at an assay dependent concentration.
|
IHC-Fr |
Use at an assay dependent concentration.
|
说明 |
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WB
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
靶标
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功能
Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. -
组织特异性
Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin. -
疾病相关
Keratoderma, palmoplantar, striate 2
Cardiomyopathy, dilated, with woolly hair and keratoderma
Arrhythmogenic right ventricular dysplasia, familial, 8
Skin fragility-woolly hair syndrome
Epidermolysis bullosa, lethal acantholytic
Cardiomyopathy, dilated, with woolly hair, keratoderma, and tooth agenesis -
序列相似性
Belongs to the plakin or cytolinker family.
Contains 17 plectin repeats.
Contains 1 SH3 domain.
Contains 6 spectrin repeats. -
结构域
Its association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins.
The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1.
The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments. -
翻译后修饰
Ser-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments.
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (PubMed:9651377). -
细胞定位
Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane. Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network (PubMed:12802069). Localizes at the intercalated disk in cardiomyocytes (By similarity). - Information by UniProt
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数据库链接
- Entrez Gene: 420869 Chicken
- Entrez Gene: 514360 Cow
- Entrez Gene: 1832 Human
- Entrez Gene: 109620 Mouse
- Entrez Gene: 306871 Rat
- Omim: 125647 Human
- SwissProt: P15924 Human
- SwissProt: E9Q557 Mouse
see all -
别名
- 250/210 kDa paraneoplastic pemphigus antigen antibody
- DCWHKTA antibody
- Desmoplakin (DPI DPII) antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab16434 被引用在 31 文献中.
- Fraichard C et al. Protease Inhibitor Anti-HIV, Lopinavir, Impairs Placental Endocrine Function. Int J Mol Sci 22:N/A (2021). PubMed: 33445576
- Ticiani E et al. Bisphenol S and Epidermal Growth Factor Receptor Signaling in Human Placental Cytotrophoblasts. Environ Health Perspect 129:27005 (2021). PubMed: 33605785
- Wong NR et al. Resident cardiac macrophages mediate adaptive myocardial remodeling. Immunity 54:2072-2088.e7 (2021). PubMed: 34320366
- Saxena M et al. The long non-coding RNA ET-20 mediates EMT by impairing desmosomes in breast cancer cells. J Cell Sci 134:N/A (2021). PubMed: 34633031
- Fraichard C et al. Placental production of progestins is fully effective in villous cytotrophoblasts and increases with the syncytiotrophoblast formation. Mol Cell Endocrinol 499:110586 (2020). PubMed: 31539598