Anti-Desmoglein 3抗体[5G11] (ab14416)

概述

  • 产品名称
    Anti-Desmoglein 3抗体[5G11]
    参阅全部 Desmoglein 3 一抗
  • 描述
    小鼠单克隆抗体[5G11] to Desmoglein 3
  • 特异性
    Reacts specifically with human Desmoglein 3 protein. Does not crossreact with Desmoglein 1 or Desmoglein 2.
  • 经测试应用
    适用于: IP, ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment (Human Desmoglein 3 extracellular domain).

  • 阳性对照
    • IHC-P: FFPE human skin melanoma tissue sections.
  • 常规说明

    The clone number has been updated from 3G133 to 5G11 since both name the same clone and 5G11 is the original clone number.

    For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

性能

应用

Our Abpromise guarantee covers the use of ab14416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 119.9 kDa.

靶标

  • 功能
    Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
  • 组织特异性
    Epidermis, tongue, tonsil, esophagus and carcinomas.
  • 序列相似性
    Contains 4 cadherin domains.
  • 结构域
    Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
  • 细胞定位
    Cell membrane. Cell junction > desmosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 130 kD pemphigus vulgaris antigen antibody
    • 130 kDa pemphigus vulgaris antigen antibody
    • Balding antibody
    • Cadherin family member 6 antibody
    • CDHF 6 antibody
    • CDHF6 antibody
    • Desmoglein 3 antibody
    • Desmoglein 3 precursor antibody
    • Desmoglein-3 antibody
    • Desmoglein3 antibody
    • DKFZp686P23184 antibody
    • DSG 3 antibody
    • DSG3 antibody
    • DSG3_HUMAN antibody
    • Pemphigus vulgaris antigen antibody
    • PVA antibody
    see all

Anti-Desmoglein 3 antibody [5G11] 图像

  • IHC image of Desmoglein 3 staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemical analysis of Human tongue tissue, staining Desmoglein 3 with ab14416.

    Tissue was fixed with formaldehyde and antigen retrieval was by proteinase K. Samples were incubated with primary antibody (1/500 in Tris buffered saline with 0.1% Tween 20) for 18 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG (1/2) was used as the secondary antibody.

    See Abreview

Anti-Desmoglein 3 antibody [5G11] (ab14416)参考文献

ab14416 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 May 25 2015

Gracias por contactarnos.

En un vial conteniendo aproximadamente 100ul de anticuerpo están contenidos 50ug de inmunoglobulina a una concentración de 0.5 mg/ml.

Espero que esta información os aclare la pregunta. ...

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Thank you for contacting us.

xxxxxxxxx is currently out of the office so I have taken over this case.

With regards to your question, a clone number is given to an antibody produced by a single clone of hybridoma cells. Each hybrido...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tongue)
Specification
Tongue
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinase K
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Nov 20 2012

DISCOUNT CODE: *** Expiration date: February 7th, 2011 I am very pleased to hear you would like to accept our offer and test ab14416 in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, ple...

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Thank you for your enquiry. There is a very helpful protocol on our website for IHC in paraffin embedded sections that will answer your questions about how to prepare the tissue sections. The protocol can be found by clicking on the Resources tab on th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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