重组Anti-Desmoglein 2/DSG2抗体[EPR6768] (ab150372)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6768] to Desmoglein 2/DSG2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Desmoglein 2/DSG2抗体[EPR6768]
参阅全部 Desmoglein 2/DSG2 一抗 -
描述
兔单克隆抗体[EPR6768] to Desmoglein 2/DSG2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HAP1, HepG2, HeLa, Human fetal heart, mouse heart and rat heart lysates. IHC-P: Human thyroid gland papillary carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6768 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab150372于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 122 kDa.
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IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 122 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
组织特异性
All of the tissues tested and carcinomas. -
疾病相关
Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10) [MIM:610193]; also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB) [MIM:612877]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
序列相似性
Contains 4 cadherin domains. -
结构域
Calcium may be bound by the cadherin-like repeats. -
细胞定位
Cell membrane. Cell junction > desmosome. - Information by UniProt
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数据库链接
- Entrez Gene: 1829 Human
- Entrez Gene: 13511 Mouse
- Entrez Gene: 307562 Rat
- Omim: 125671 Human
- SwissProt: Q14126 Human
- SwissProt: O55111 Mouse
- Unigene: 412597 Human
- Unigene: 345891 Mouse
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别名
- ARVC 10 antibody
- ARVC10 antibody
- ARVD 10 antibody
see all
图片
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All lanes : Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : DSG2 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 90-160 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab150372 observed at 90-160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab150372 was shown to react with Desmoglein 2/DSG2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261826 (knockout cell lysate ab257158) was used. Wild-type HeLa and DSG2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab150372 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Desmoglein 2/DSG2 with ab150372 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) at 1/10000 dilution
Lane 1 : Trypsinised HAP1 cell lysate
Lane 2 : Scraped HAP1 cell lysate
Lane 3 : Trypsinised DSG2 knockout HAP1 cell lysate
Lane 4 : Scraped DSG2 knockout HAP1 cell lysate
Lane 5 : Trypsinised A431 cell lysate
Lane 6 : Scraped A431 cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Lanes 1 - 6: Merged signal (red and green). Green - ab150372 observed at 95 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab150372 was shown to react with Desmoglein 2 in wild-type HAP1 cells in western blot with loss of signal observed in DSG2 knockout sample. Wild-type and DSG2 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab150372 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal heart lysate
Lane 4 : mouse heart lysate
Lane 5 : rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 122 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab150372 被引用在 17 文献中.
- Vallverdú-Prats M et al. Premature Termination Codon in 5' Region of Desmoplakin and Plakoglobin Genes May Escape Nonsense-Mediated Decay through the Reinitiation of Translation. Int J Mol Sci 23:N/A (2022). PubMed: 35054841
- Teng ACT et al. Tmem65 is critical for the structure and function of the intercalated discs in mouse hearts. Nat Commun 13:6166 (2022). PubMed: 36257954
- Yang L et al. Immune Profiling Reveals Molecular Classification and Characteristic in Urothelial Bladder Cancer. Front Cell Dev Biol 9:596484 (2021). PubMed: 33777927
- Qin S et al. DSG2 expression is correlated with poor prognosis and promotes early-stage cervical cancer. Cancer Cell Int 20:206 (2020). PubMed: 32514251
- Chen X et al. UPLC-Q-TOF/MS-Based Serum and Urine Metabonomics Study on the Ameliorative Effects of Palmatine on Helicobacter pylori-Induced Chronic Atrophic Gastritis. Front Pharmacol 11:586954 (2020). PubMed: 33041831