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Full length native protein (purified) (Human).
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab150372 as a replacement.
Our Abpromise guarantee covers the use of ab14415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This image is courtesy of an anonymous Abreview
The blot was blocked with 5% milk for 1 hour at 21°C and incubated with the primary antibody for 12 hours at 4°C.
The antibody detects a species of the predicted mobility (125 kDa) in Human cell lines U2OS (1) and 293T (2). No signal was observed in extracts from other species, suggesting that this antibody does not cross-react with AGMk, Hm, Ms or Rat.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"