Anti-Desmoglein 2抗体[3G132] (ab14415)

概述

  • 产品名称Anti-Desmoglein 2抗体[3G132]
    参阅全部 Desmoglein 2 一抗
  • 描述
    小鼠单克隆抗体[3G132] to Desmoglein 2
  • 特异性This antibody does not cross-react with Desmoglein 1 or Desmoglein 3. The epitope is within the extracellular domain of Desmoglein 2.
  • 经测试应用适用于: IP, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) (Human).

  • 常规说明For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

性能

应用

Our Abpromise guarantee covers the use of ab14415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
WB
ICC/IF
IHC-P
  • 应用说明IF: Use at a concentration of 20 - 50 µg/ml.
    IHC-P: Used at a dilution of 1/10 for 30 min on human bladder cancer (see Abreview).
    IP: Use at a concentration of 5 - 10 µg/ml.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 165 kDa (predicted molecular weight: 122 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
    • 组织特异性All of the tissues tested and carcinomas.
    • 疾病相关Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10) [MIM:610193]; also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
      Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB) [MIM:612877]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • 序列相似性Contains 4 cadherin domains.
    • 结构域Calcium may be bound by the cadherin-like repeats.
    • 细胞定位Cell membrane. Cell junction > desmosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ARVC 10 antibody
      • ARVC10 antibody
      • ARVD 10 antibody
      • ARVD10 antibody
      • Cadherin family member 5 antibody
      • CDHF 5 antibody
      • CDHF5 antibody
      • CMD1BB antibody
      • Desmoglein 2 antibody
      • Desmoglein-2 antibody
      • Desmoglein2 antibody
      • DSG 2 antibody
      • DSG2 antibody
      • DSG2_HUMAN antibody
      • HDGC antibody
      • HDGC included antibody
      • Human Desmoglein colon antibody
      • MGC117034 antibody
      • MGC117036 antibody
      • MGC117037 antibody
      see all

    Anti-Desmoglein 2 antibody [3G132] 图像

    • ab14415 at a 1/10 dilution staining human urothelium (bladder cancer) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes following heat mediated antigen retrieval and paraformaldehyde fixation.

      See Abreview

    • All lanes : Anti-Desmoglein 2 antibody [3G132] (ab14415) at 3 µg/ml

      Lane 1 : U2OS (Hu) whole cell lysate
      Lane 2 : 293T (Hu) whole cell lysate
      Lane 3 : Cos-7 (AGMk) whole cell lysate
      Lane 4 : CHO (Hm) whole cell lysate
      Lane 5 : PC12 (Rat) whole cell lysate
      Lane 6 : 3T3 (Ms) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      HRP conjugated goat anti-mouse antibody
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 122 kDa
      Observed band size : 125 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes

      This image is courtesy of an anonymous Abreview

      See Abreview

    Anti-Desmoglein 2 antibody [3G132] (ab14415)参考文献

    This product has been referenced in:
    • Ebert LM  et al. A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis. Angiogenesis 19:463-86 (2016). IHC-P ; Mouse . Read more (PubMed: 27338829) »
    • Galoian K  et al. Restoration of desmosomal junction protein expression and inhibition of H3K9-specific histone demethylase activity by cytostatic proline-rich polypeptide-1 leads to suppression of tumorigenic potential in human chondrosarcoma cells. Mol Clin Oncol 3:171-178 (2015). Human . Read more (PubMed: 25469290) »

    See all 5 Publications for this product

    Product Wall

    The epitope for ab14415 is located on the extracellular domain.

    With A431 cell lysate, this antibody detects a band at ˜165 kDa (represents apparent MW with post-translational modification; amino acid backbone has a calculated MW of ˜122 kDa...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (salivary gland)
    Specification salivary gland
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Tris pH=7.4 / 1mM EDTA
    Permeabilization No
    Username

    Abcam user community

    Verified customer

    提交于 Jan 02 2013

    Thank you for your reply.

    I can confirm that this antibody does not cross-react with DSG1 or DSG3. Based on the information available on the other DSG2 antibodies, this is the only one I can confirm has been tested for such cross-reactivity....

    Read More

    Thank you for contacting Abcam regarding ab14415.


    I have run a sequence alignment of the human protein with the mouse sequence and found overall 76% identity. The specific epitope has not been mapped, thus it is difficult to determine i...

    Read More

    Vielen Dank für Ihre Geduld. Ich kann Ihnen jetzt auch bestätigen, dass der ab14415 ein Epitop auf dem extrazellulären Part des Desmoglein 2 erkennt. Auch der ab96761 erkennt das extrazelluläre Desmoglein 2, da der Antikörper gegen ein rekombina...

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    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten. Ich habe das Labor angefragt, ob etwas bekannt ist im Bezug für das Epitops des ab14415 und des ab96761. Ich werde Sie wieder kontaktieren sobald ich eine Antwort bekommen haben. ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (293T)
    Total protein in input 250 µg
    Specification 293T
    Immuno-precipitation step Protein G
    Username

    Abcam user community

    Verified customer

    提交于 Dec 03 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (U2OS and 293T cell line)
    Loading amount 30 µg
    Specification U2OS and 293T cell line
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 02 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Bladder cancer , normal urothelium)
    Specification Bladder cancer , normal urothelium
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated
    Username

    Mr. Rudolf Jung

    Verified customer

    提交于 Aug 07 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"