RabMAb

Anti-Desmoglein 1抗体[EPR6766(B)] (ab124798)

概述

  • 产品名称Anti-Desmoglein 1抗体[EPR6766(B)]
    参阅全部 Desmoglein 1 一抗
  • 描述
    兔单克隆抗体[EPR6766(B)] to Desmoglein 1
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues at the C-terminal of Human Desmoglein 1 (Q02413).

  • 阳性对照
    • Human skin lysate, Human skin tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 114 kDa.
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
  • 应用说明Is unsuitable for ICC or IP.
  • 靶标

    • 功能Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
    • 组织特异性Epidermis, tongue, tonsil and esophagus.
    • 疾病相关Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
    • 序列相似性Contains 4 cadherin domains.
    • 结构域Calcium may be bound by the cadherin-like repeats.
    • 细胞定位Cell membrane. Cell junction > desmosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cadherin family member 4 antibody
      • CDHF4 antibody
      • Desmoglein-1 antibody
      • Desmosomal glycoprotein 1 antibody
      • DG1 antibody
      • DGI antibody
      • DSG antibody
      • DSG1 antibody
      • DSG1_HUMAN antibody
      • EPKHE antibody
      • EPKHIA antibody
      • Pemphigus foliaceus antigen antibody
      • PPKS1 antibody
      • SPPK1 antibody
      see all

    Anti-Desmoglein 1 antibody [EPR6766(B)] 图像

    • Anti-Desmoglein 1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 114 kDa
    • ab124798, at a 1/250 dilution, staining Desmoglein 1 in paraffin embedded Human skin tissue by Immunohistochemistry.

    Anti-Desmoglein 1 antibody [EPR6766(B)] (ab124798)参考文献

    ab124798 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (skin)
    Specification skin
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative Acetone
    Username

    Abcam user community

    Verified customer

    提交于 Mar 24 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Sample Mouse Tissue sections (epidermis)
    Specification epidermis
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 May 29 2013

    Abreviews
    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Sample Mouse Tissue sections (Epidermis)
    Specification Epidermis
    Permeabilization No
    Fixative Methanol
    Username

    Abcam user community

    Verified customer

    提交于 May 29 2013

    Western blot

    Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing
    Sample Mouse Tissue lysate - whole (epidermis)
    Specification epidermis
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 May 29 2013

    Thank you for contacting us. Although we do not have DSG 1 and 3 antibodies that have been tested for reactivity with horse or even in all the applications you anticipate using, we do have at least one DSG1 antibody that I think will be useful.
    <...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"