重组
RabMAb

Anti-Desmoglein 1抗体[EPR6766(B)] (ab124798)

概述

  • 产品名称
    Anti-Desmoglein 1抗体[EPR6766(B)]
    参阅全部 Desmoglein 1 一抗
  • 描述
    兔单克隆抗体[EPR6766(B)] to Desmoglein 1
  • 经测试应用
    适用于: WB, IHC-Pmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues at the C-terminal of Human Desmoglein 1 (Q02413).

  • 阳性对照
    • Human skin lysate, Human skin tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 114 kDa.
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
  • 应用说明
    Is unsuitable for ICC or IP.
  • 靶标

    • 功能
      Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
    • 组织特异性
      Epidermis, tongue, tonsil and esophagus.
    • 疾病相关
      Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
    • 序列相似性
      Contains 4 cadherin domains.
    • 结构域
      Calcium may be bound by the cadherin-like repeats.
    • 细胞定位
      Cell membrane. Cell junction > desmosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cadherin family member 4 antibody
      • CDHF4 antibody
      • Desmoglein-1 antibody
      • Desmosomal glycoprotein 1 antibody
      • DG1 antibody
      • DGI antibody
      • DSG antibody
      • DSG1 antibody
      • DSG1_HUMAN antibody
      • EPKHE antibody
      • EPKHIA antibody
      • Pemphigus foliaceus antigen antibody
      • PPKS1 antibody
      • SPPK1 antibody
      see all

    Anti-Desmoglein 1 antibody [EPR6766(B)] 图像

    • Anti-Desmoglein 1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 114 kDa
    • ab124798, at a 1/250 dilution, staining Desmoglein 1 in paraffin embedded Human skin tissue by Immunohistochemistry.

    Anti-Desmoglein 1 antibody [EPR6766(B)] (ab124798)参考文献

    This product has been referenced in:
    • Quispe Calla NE  et al. Medroxyprogesterone acetate and levonorgestrel increase genital mucosal permeability and enhance susceptibility to genital herpes simplex virus type 2 infection. Mucosal Immunol 9:1571-1583 (2016). IHC-P ; Mouse . Read more (PubMed: 27007679) »

    See 1 Publication for this product

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    提交于 Mar 24 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Sample
    Mouse Tissue sections (epidermis)
    Specification
    epidermis
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 May 29 2013

    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Sample
    Mouse Tissue sections (Epidermis)
    Specification
    Epidermis
    Permeabilization
    No
    Fixative
    Methanol
    Username

    Abcam user community

    Verified customer

    提交于 May 29 2013

    Western blot

    Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (epidermis)
    Specification
    epidermis
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 May 29 2013

    Thank you for contacting us. Although we do not have DSG 1 and 3 antibodies that have been tested for reactivity with horse or even in all the applications you anticipate using, we do have at least one DSG1 antibody that I think will be useful.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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